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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.12
- 수록면
- 270 - 276 (7page)
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초록· 키워드
오류제보하기
In glycoprotein, Terminal sialic acid residues of Nlinked glycan are imperative things because they prevent the recognition from asialoglycoprotein-receptor that affect the half-life of glycoproteins. So establishment of culture process for enhancing sialic acid is important to maximize sialic acid contents of glycoprotein. In this study, we investigated effects of biphasic culture of Chinese hamster ovary (CHO) cells producing albumin-erythropoietin to increase sialylation. Biphasic cultures were performed with shift of CO₂ concentrations and temperatures at day 5 when viable cell density was decreased and sialidase was started to be released by cell lysis. The examined temperature set points were 33, 35 and 37℃ respectively and the CO₂ concentration was 1, 5, 10 and 15%. We confirmed that sialidase activity was the lowest in biphasic culture that was shifted from normal culture condition to 1% of CO₂ and 33℃ on day 5. However, the temperature and concentration of CO₂ have little effect on activity of α2,3-sialyltransferase. Also, sialic acid contents were enhanced 1.13-fold higher than that in control culture. In conclusion, Biphasic cultivation in CHO cells led to inhibition of sialidase activity and increases of sialylated glycan.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (18)
참고문헌 신청
[학술지(정기간행물)] - Jayapal, K. P. / 2007 / Recombinant protein therapeutics from CHO Cells - 20 years and counting / Chem. Eng. Prog 103 : 40 ~ 47
[학술지(정기간행물)] - Xu, X. / 2011 / The genomic sequence of the Chinese hamster ovary (CHO)-K1cell line / Nat. Biotechnol 29 : 735 ~ 741
[학술지(정기간행물)] - Lingg, N. / 2012 / The sweet tooth of biopharmaceuticals: Importance of recombinant protein glycosylation analysis / Biotechnol. J 7 : 1462 ~ 1472
[학술지(정기간행물)] - Kaisermayer, C. / 2016 / Biphasic cultivation strategy to avoid Epo-Fc aggregation and optimize protein expression / J. Biotechnol 227 : 3 ~ 9
[학술지(정기간행물)] - Schmidberger, T. / 2015 / Progress toward forecasting product quality and quantity of mammalian cell culture processes by performance-based modeling / Biotechnol. Prog 31 : 1119 ~ 1127
[학술지(정기간행물)] - Xu, X. / 2011 / The genomic sequence of the Chinese hamster ovary (CHO)-K1cell line / Nat. Biotechnol 29 : 735 ~ 741
[학술지(정기간행물)] - Lingg, N. / 2012 / The sweet tooth of biopharmaceuticals: Importance of recombinant protein glycosylation analysis / Biotechnol. J 7 : 1462 ~ 1472
[학술지(정기간행물)] - Kaisermayer, C. / 2016 / Biphasic cultivation strategy to avoid Epo-Fc aggregation and optimize protein expression / J. Biotechnol 227 : 3 ~ 9
[학술지(정기간행물)] - Schmidberger, T. / 2015 / Progress toward forecasting product quality and quantity of mammalian cell culture processes by performance-based modeling / Biotechnol. Prog 31 : 1119 ~ 1127
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UCI(KEPA) : I410-ECN-0101-2017-470-001981627