지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한외과학회 Annals of Surgical Treatment and Research Annals of Surgical Treatment and Research Vol.91 No.6
- 발행연도
- 2016.12
- 수록면
- 273 - 277 (5page)
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초록· 키워드
오류제보하기
Purpose: Livin is associated with drug response in several cancers. The aim of this study was to investigate the effect of silencing the livin gene expression on anticancer drug response in colorectal cancer.
Methods: siRNA was transfected at different concentrations (0, 10, and 30nM) into HCT116 cells, then cells were treated with either 5-fluorouracil (FU)/leucovorin (LV) or oxaliplatin (L-OHP)/5-FU/LV. Cellular viability and apoptosis were evaluated following silencing of livin gene expression combined with treatment with anticancer drugs.
Results: Livin gene expression was effectively suppressed by 30nM siRNA compared with control and 10nM siRNA. The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay showed that proliferation was effectively inhibited in cells treated with a combination of both siRNA and an anticancer drug, compared to cells treated with siRNA-Livin or anticancer drug alone. In particular, the combination of 30nM siRNA and L-OHP/5-FU/LV resulted in a 93.8% and 91.4% decrease, compared to untreated control or L-OHP/5-FU/LV alone, respectively. Cellular proliferation was most effectively suppressed by a combination of 30nM of siRNA and L-OHP/5-FU/LV compared to other combinations.
Conclusion: siRNA-mediated down-regulation of livin gene expression could significantly suppress colon cancer growth and enhance the cytotoxic effects of anticancer drugs such as 5-FU and L-OHP. The results of this study suggest that silencing livin gene expression in combination with treatment with anticancer drugs might be a novel cancer therapy for colorectal cancer.
Methods: siRNA was transfected at different concentrations (0, 10, and 30nM) into HCT116 cells, then cells were treated with either 5-fluorouracil (FU)/leucovorin (LV) or oxaliplatin (L-OHP)/5-FU/LV. Cellular viability and apoptosis were evaluated following silencing of livin gene expression combined with treatment with anticancer drugs.
Results: Livin gene expression was effectively suppressed by 30nM siRNA compared with control and 10nM siRNA. The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay showed that proliferation was effectively inhibited in cells treated with a combination of both siRNA and an anticancer drug, compared to cells treated with siRNA-Livin or anticancer drug alone. In particular, the combination of 30nM siRNA and L-OHP/5-FU/LV resulted in a 93.8% and 91.4% decrease, compared to untreated control or L-OHP/5-FU/LV alone, respectively. Cellular proliferation was most effectively suppressed by a combination of 30nM of siRNA and L-OHP/5-FU/LV compared to other combinations.
Conclusion: siRNA-mediated down-regulation of livin gene expression could significantly suppress colon cancer growth and enhance the cytotoxic effects of anticancer drugs such as 5-FU and L-OHP. The results of this study suggest that silencing livin gene expression in combination with treatment with anticancer drugs might be a novel cancer therapy for colorectal cancer.
목차
INTRODUCTION
METHODS
RESULTS
DISCUSSION
참고문헌 (23)
참고문헌 신청
de Castro-Carpeno J. / 2008 / EGFR and colon cancer : a clinical view / Clin Transl Oncol 10:6~13
오승엽 / 2015 / Oncologic outcomes following metastasectomy in colorectal cancer patients developing distant metastases after initial treatment / 대한외과학회지 88(5):253~259
Bremer E. / 2006 / Targeted induction of apoptosis for cancer therapy : current pro gress and prospects / Trends Mol Med 12:382~393
Schimmer AD / 2004 / Inhibitor of apoptosis proteins : translating basic knowledge into clini cal practice / Cancer Res 64:7183~7190
Chang H. / 2007 / Livin/melanoma inhibitor of apoptosis protein as a potential therapeutic target for the treatment of malignancy / Mol Cancer Ther 6:2430
오승엽 / 2015 / Oncologic outcomes following metastasectomy in colorectal cancer patients developing distant metastases after initial treatment / 대한외과학회지 88(5):253~259
Bremer E. / 2006 / Targeted induction of apoptosis for cancer therapy : current pro gress and prospects / Trends Mol Med 12:382~393
Schimmer AD / 2004 / Inhibitor of apoptosis proteins : translating basic knowledge into clini cal practice / Cancer Res 64:7183~7190
Chang H. / 2007 / Livin/melanoma inhibitor of apoptosis protein as a potential therapeutic target for the treatment of malignancy / Mol Cancer Ther 6:2430
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UCI(KEPA) : I410-ECN-0101-2017-514-001873103