지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
The measurement of viral load in HIV-1 infected patients is essential for the establishment of a therapeutic strategy. Several commercial assays have shown shortcomings in quantifying rare genotypes of HIV-1 such as minor groups of N and O. In this study, the HIV-1 RT-qPCR assay was developed. The primers and probe of HIV-1 were designed to target the pol gene and to increase the detection efficiency of various subtypes including group N and O. The HIV-1 quantitative RT-qPCR assay was assessed for its analytical performance and clinical evaluation. The LoD was determined to 33.9 IU/ml. The LoD of several subtypes including A, C, D, CRF_01AE, F, CRF_02AG, G and H, were determined to less than 40 IU/ml. The HIV-1 quantitative RT-qPCR assay was evaluated using the China National Reference Panel of HIV-1 RNA to determine the analytical performance. The results were all within the acceptable range. The clinical evaluation was performed at Hunan CDC in China. The clinical evaluation results were compared with those of the China domestic commercial kit. A significant correlation (fresh samples; R<SUP>2</SUP>=0.84, P＜0.001, frozen samples; R<SUP>2</SUP>=0.76, P＜0.001) between the two systems was observed for 64 fresh samples and 76 frozen samples with viral loads, and the Bland-Altman plot showed good agreement (98.4%, 96.1%, respectively). In conclusion, the HIV-1 quantitative RT-qPCR assay had comparable analytical performance with several commercial kits. The study provides basic data for the research of HIV-1 diagnosis and the development of P＜HIV-1 molecular diagnostic assay.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (34)
참고문헌 신청
[단행본] - Abbott / 2012 / Abbott RealTime HIV-1 / Abbott
[학술지(정기간행물)] - Abecasis AB / 2013 / HIV-1 subtype distribution and its demographic determinants in newly diagnosed patients in Europe suggest highly compartmentalized epidemics / Retrovirology 10 : 7 ~
[기타자료] - AIDSinfor / 2014 / Guidelines for the use of antiretroviral agents in HIVinfected adults and adolescents / ADISinfor
[학술지(정기간행물)] - Amendola A / 2002 / Underevaluation of HIV-1 plasma viral load by a commercially available assay in a cluster of patients infected with HIV-1A/G circulating recombinant form (CRF02) / J Acquir Immune Defic Syndr 31 : 488 ~ 494
[학술지(정기간행물)] - Amendola A / 2014 / Ability of two commercially available assays (Abbott RealTime HIV-1 and Cobas AmpliPrep/Cobas Taq-Man HIV-1 version 2.0) to quantify low HIV-1 RNA levels (<1,000 copies/milliliter): Comparison with clinical samples and NIBSC working reagent for nucleic aci / J Clin Microbiol 52 : 2019 ~ 2026
[학술지(정기간행물)] - Abecasis AB / 2013 / HIV-1 subtype distribution and its demographic determinants in newly diagnosed patients in Europe suggest highly compartmentalized epidemics / Retrovirology 10 : 7 ~
[기타자료] - AIDSinfor / 2014 / Guidelines for the use of antiretroviral agents in HIVinfected adults and adolescents / ADISinfor
[학술지(정기간행물)] - Amendola A / 2002 / Underevaluation of HIV-1 plasma viral load by a commercially available assay in a cluster of patients infected with HIV-1A/G circulating recombinant form (CRF02) / J Acquir Immune Defic Syndr 31 : 488 ~ 494
[학술지(정기간행물)] - Amendola A / 2014 / Ability of two commercially available assays (Abbott RealTime HIV-1 and Cobas AmpliPrep/Cobas Taq-Man HIV-1 version 2.0) to quantify low HIV-1 RNA levels (<1,000 copies/milliliter): Comparison with clinical samples and NIBSC working reagent for nucleic aci / J Clin Microbiol 52 : 2019 ~ 2026
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UCI(KEPA) : I410-ECN-0101-2017-510-001392576