본 연구는 온도, 광세기 및 pH가 Chlorella vulgaris (C. vulgaris)의 증식률에 미치는 영향을 알아보고자 하였다. 각 조건에서의 효율을 평가하기 위하여 C. vulgaris (FC-16) (3~8 μm)를 Jaworski`s Medium에 증식시킨 뒤, 다양한 온도(10℃, 15℃, 20℃, 25℃, 30℃, 35℃)와 다양한 광세기(100~800 μEm-2s-1) 및 다양한 pH (3, 4, 5, 6, 7, 7.5, 9) 조건에서 실험하였다. 실험결과, 25~30℃ 조건에서는 10℃ 조건과 비교 시 같은 양의 C. vulgaris를 증식하는데 걸리는 시간은 약 5.2~5.5배 빨랐고, Chlorophyll a는 5~5.5배 높았으며, 단위 면적당 cell volume은 14% 이상 높아 C. vulgaris 최적 배양온도는 25~30℃로 조사되었다. C. vulgaris 배양기간의 경우, 증식속도가 5일까지는 느리게 증가했지만, 6일 이후부터 15일까지는 폭발적으로 증가했고, 15일 이후에는 거의 증식이 멈춰 15일 이내가 적당한 것을 알 수 있었다. C. vulgaris 증식을 위한 최적 pH는 pH 7~7.5로 조사되었다.
The aim of this study was to investigate the efficiency of temperature, light intensity and pH on the growth rate of Chlorella vulgaris (C. vulgaris). The size of C. vulgaris (FC-16) was 3-8 μm, having round in shape. The cells of C. vulgaris (FC-16) was cultured in the Jaworski`s Medium with deionized water. To evaluate the efficiency of temperature, light intensity and pH on the growth rate of C. vulgaris, six different fractions of temperature (10℃, 15℃, 20℃, 25℃, 30℃, 35℃), various light intensities (100-800 μEm-2s-1) and seven different fractions of pH (3, 4, 5, 6, 7, 7.5, 9) were prepared. The growth rate of C. vulgaris cultivation was approximately 5.2 to 5.5 times faster, the concentration of Chlorophyll a was also 5 to 5.5 times higher, and cell volume per unit area was 14% higher at 25℃ to 30℃ than those at 10℃. Therefore, the optimal temperature for cultivation of C. vulgaris was estimated 25℃ to 30℃. The growth rate of C. vulgaris increased slowly up to 5 days, exploded after 5 days until 15 days, and then stoped after that. The optimum cultivation period of C. vulgaris was estimated as 15 days. The optimum pH for the growth rate of C. vulgaris was determined pH 7 to 7.5.