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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.6
- 수록면
- 113 - 119 (7page)
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초록· 키워드
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A novel agar-degrading marine bacterium, SH-1 strain, was isolated from seashore of Namhae at Gyeongnam province, Korea. The SH-1 strain exhibited 98% similarity with Alteromonas species based on 16S rDNA sequencing and named as Alteromonas sp. SH-1. Alteromonas sp. SH-1 showed agarase activity of 348.3 U/L (1.67 U/mg protein). The molecular masses of the enzymes were predicted as about 85 kDa and 110 kDa by SDS-PAGE and zymogram. The enzymatic activity was optimal at 30℃ and the relative agarase activity was decreased as temperature increase from 30℃ and thus about 90% and 70% activities were shown at 40oC and 50℃, respectively. The optimum pH was 6.0 for agarase activity in 20 mM Tris-HCl buffer and activities were less than 70% and 85% activity at pH 5.0 and pH 7.0, respectively, compared with that at pH 6. Agarase activity has remained over 90% at 20℃ after 1.5 hour exposure at this temperature. However, its activity was less than 60% at 30℃ after 0.5 h exposure at this temperature. The enzymes produced agarooligosaccharides such as agaropentaose and agarotriose from agarose, indicating that the agarases are α-agarases. Thus, Alteromonas sp. SH-1 and its agarases would be useful for the industrial production of agarooligosaccharides which are known as having anticancer and antioxidation activities.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (23)
참고문헌 신청
[학술지(정기간행물)] - 장혜지 / 2011 / 해양성 Flammeovirga sp. mbrc-1 균주의 분리 및 한천분해기능의 특성조사 / KSBB Journal 26 (6) : 552 ~ 556
[학술지(정기간행물)] - 이상현 / 2007 / 한천분해효소를 생산하는 해양세균 Thalassomonas sp. SL-5의 분리 및 특성 / 생명과학회지 17 (2) : 70 ~ 75
[학술지(정기간행물)] - Hong, J. H. / 2001 / Effect of agarooligosaccharides on the growth of intestinal bacteria / J. Fd. Hyg. Safety 16 : 11 ~ 15
[학술지(정기간행물)] - Seo, Y. B. / 2014 / Heterologous expression of a newly screened β-agarase from Alteromonas sp. GNUM1 in Escherichia coli and its application for agarose degradation / Process Biochem 49 : 430 ~ 436
[학술지(정기간행물)] - Yang, M. / 2014 / Purification and characterization of two agarases from Agarivorans albus OAY 02 / Process Biochem 49 : 905 ~ 912
[학술지(정기간행물)] - 이상현 / 2007 / 한천분해효소를 생산하는 해양세균 Thalassomonas sp. SL-5의 분리 및 특성 / 생명과학회지 17 (2) : 70 ~ 75
[학술지(정기간행물)] - Hong, J. H. / 2001 / Effect of agarooligosaccharides on the growth of intestinal bacteria / J. Fd. Hyg. Safety 16 : 11 ~ 15
[학술지(정기간행물)] - Seo, Y. B. / 2014 / Heterologous expression of a newly screened β-agarase from Alteromonas sp. GNUM1 in Escherichia coli and its application for agarose degradation / Process Biochem 49 : 430 ~ 436
[학술지(정기간행물)] - Yang, M. / 2014 / Purification and characterization of two agarases from Agarivorans albus OAY 02 / Process Biochem 49 : 905 ~ 912
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UCI(KEPA) : I410-ECN-0101-2017-470-000801984