Optimization of Trichomonas vaginalis Diagnosis during Pregnancy at a University Hospital, Argentina

Pamela Testardini; María Lucía Gallo Vaulet; Andrea Carolina Entrocassi; Claudia Menghi; Martha Cora Eliseht; Claudia Gatta; Mirta Losada; María Sol Touzón; Ana Corominas; Carlos Vay; Silvio Tatti; Angela Famiglietti; Marcelo Rodriguez Fermepin; Beatriz Perazzi

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저자정보: Pamela Testardini (Universidad de Buenos Aires) María Lucía Gallo Vaulet (Universidad de Buenos Aires) Andrea Carolina Entrocassi (Universidad de Buenos Aires) Claudia Menghi (Universidad de Buenos Aires) Martha Cora Eliseht (Universidad de Buenos Aires) Claudia Gatta (Universidad de Buenos Aires) Mirta Losada (Universidad de Buenos Aires) María Sol Touzón (Universidad de Buenos Aires) Ana Corominas (Universidad de Buenos Aires) Carlos Vay (Universidad de Buenos Aires) Silvio Tatti (Universidad de Buenos Aires) Angela Famiglietti (Universidad de Buenos Aires) Marcelo Rodriguez Fermepin (Universidad de Buenos Aires) Beatriz Perazzi (Universidad de Buenos Aires)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.54 No.2

발행연도: 2016.4

수록면: 191 - 195 (5page)

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The aim of this study was to evaluate different methods for Trichomonas vaginalis diagnosis during pregnancy in order to prevent maternal and perinatal complications. A total of 386 vaginal exudates from pregnant women were analyzed. T. vaginalis was investigated by 3 types of microscopic examinations direct wet mount with physiologic saline solution, prolonged May-Grunwald Giemsa (MGG) staining, and wet mount with sodium-acetate-formalin (SAF)/methylene blue method. PCR for 18S rRNA gene as well as culture in liquid medium were performed. The sensitivity and specificity of the microscopic examinations were evaluated considering the culture media positivity or the PCR techniques as gold standard. The frequency of T. vaginalis infection was 6.2% by culture and/or PCR, 5.2% by PCR, 4.7% by culture, 3.1% by SAF/methylene blue method and 2.8% by direct wet smear and prolonged MGG staining. The sensitivities were 83.3%, 75.0%, 50.0%, and 45.8% for PCR, culture, SAF/methylene blue method, and direct wet smear-prolonged MGG staining, respectively. The specificity was 100% for all the assessed methods. Microscopic examinations showed low sensitivity, mainly in asymptomatic pregnant patients. It is necessary to improve the detection of T. vaginalis using combined methods providing higher sensitivity, such as culture and PCR, mainly in asymptomatic pregnant patients, in order to prevent maternal and perinatal complications.

#Trichomonas vaginalis #diagnosis #pregnancy #Argentina

참고문헌 (21)

참고문헌 신청

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