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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.2
- 수록면
- 109 - 112 (4page)
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초록· 키워드
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We performed a molecular genetic study on the sequences of 18S ribosomal RNA (ITS1 region) gene in 4-day-old adult worms of Macroorchis spinulosus recovered in mice experimentally infected with metacercariae from crayfish in Jeollanam-do Province, Korea. The metacercariae were round, 180 μm in average diameter, encysted with 2 layers of thick walls, but the stylet on the oral sucker was not clearly seen. The adult flukes were oval shape, and 760-820 μm long and 320-450 μm wide, with anterolateral location of 2 large testes. The phylogenetic tree based on ITS1 sequences of 6 M. spinulosus samples showed their distinguished position from other trematode species in GenBank. The most closely resembled group was Paragonimus spp. which also take crayfish or crabs as the second intermediate host. The present study is the first molecular characterization of M. spinulosus and provided a basis for further phylogenetic studies to compare with other trematode fauna in Korea.
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참고문헌 (8)
참고문헌 신청
Ando A. On a new trematode with the freshwater crab as its intermediate host. Tokyo Iji Shinshi 1918; no. 2081: 10-17 (in Japanese).
Chai JY, Sohn WM, Huh S, Choi MH, Lee SH. Redescription of Macroorchis spinulosus Ando, 1918 (Digenea: Nanophyetidae) encysted in the fresh water crayfish, Cambaroides similis. Korean J Parasitol 1996; 34: 1-6.
Machida M, Uchida A. Some helminth parasites of the Japanese shrew mole from the Izu Peninsula. Memo Nat Sci Museum 1982; 15: 149-154.
Saito Y, Watanabe T, Yamashita T. Two new intestinal trematodes, Macroorchis chimarrogalus n. sp. and Macroorchis elongatus n. sp. from Japanese water shrew and others, with a description of the metacercaria of Macroorchis chimarrogalus n. sp. (Trematoda: Nanophyetidae). Acta Med Biol 1982; 30: 47-56.
Luton K, Walker D, Blair D. Comparison of ribosomal internal transcribed spacers from two congeneric species of flukes (Platyhelminths: Trematoda: Digenea). Mol Biochem Parasitol 1992; 56: 323-328.
Chai JY, Sohn WM, Huh S, Choi MH, Lee SH. Redescription of Macroorchis spinulosus Ando, 1918 (Digenea: Nanophyetidae) encysted in the fresh water crayfish, Cambaroides similis. Korean J Parasitol 1996; 34: 1-6.
Machida M, Uchida A. Some helminth parasites of the Japanese shrew mole from the Izu Peninsula. Memo Nat Sci Museum 1982; 15: 149-154.
Saito Y, Watanabe T, Yamashita T. Two new intestinal trematodes, Macroorchis chimarrogalus n. sp. and Macroorchis elongatus n. sp. from Japanese water shrew and others, with a description of the metacercaria of Macroorchis chimarrogalus n. sp. (Trematoda: Nanophyetidae). Acta Med Biol 1982; 30: 47-56.
Luton K, Walker D, Blair D. Comparison of ribosomal internal transcribed spacers from two congeneric species of flukes (Platyhelminths: Trematoda: Digenea). Mol Biochem Parasitol 1992; 56: 323-328.
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UCI(KEPA) : I410-ECN-0101-2016-513-002569550