지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.12
- 수록면
- 45 - 52 (8page)
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초록· 키워드
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Objectives : The present study was designed to systematically characterize the denaturation and the renaturation of double stranded DNA (dsDNA), which is suitable for DNA hybridization.
Methods : A series of physical and chemical denaturation methods were implemented on well-defined 86-bp dsDNA fragment. The degree of each denaturation was measured and the most suitable denaturation method was determined. DNA renaturation tendency was also investigated for the suggested denaturation method.
Results : Heating, beads mill, and sonication bath did not show any denaturation for 30 minutes. However probe sonication fully denatured DNA in 5 minutes. 1 mol/L sodium hydroxide (alkaline treatment) and 60% dimethyl sulfoxide (DMSO) treatment fully denatured DNA in 2-5 minutes.
Conclusions : Among all the physical methods applied, the direct probe sonication was the most effective way to denature the DNA fragments. Among chemical methods, 60% DMSO was the most adequate denaturation method since it does not cause full renaturation during DNA hybridization.
Methods : A series of physical and chemical denaturation methods were implemented on well-defined 86-bp dsDNA fragment. The degree of each denaturation was measured and the most suitable denaturation method was determined. DNA renaturation tendency was also investigated for the suggested denaturation method.
Results : Heating, beads mill, and sonication bath did not show any denaturation for 30 minutes. However probe sonication fully denatured DNA in 5 minutes. 1 mol/L sodium hydroxide (alkaline treatment) and 60% dimethyl sulfoxide (DMSO) treatment fully denatured DNA in 2-5 minutes.
Conclusions : Among all the physical methods applied, the direct probe sonication was the most effective way to denature the DNA fragments. Among chemical methods, 60% DMSO was the most adequate denaturation method since it does not cause full renaturation during DNA hybridization.
목차
Introduction
Materials and Methods
Results
Discussion
References
참고문헌 (32)
참고문헌 신청
Li S / 2010 / A novel automated assay with dual-color hybridization for single-nucleotide polymorphisms genotyping on gold magnetic nanoparticle array / Anal Biochem 405(1):141~143
Sturtevant JM / 1958 / The heat of denaturation of DNA / J Am Chem Soc 80(11):2911
Ando T / 1966 / A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae / Biochim Biophys Acta 114(1):158~168
Markarian SA / 2006 / Effect of diethylsulfoxide on the thermal denaturation of DNA / Biopolymers 82(1):1~5
Yu ZW / 1994 / Dimethyl sulphoxide: a review of its applications in cell biology / Biosci Rep 14(6):259~281
Sturtevant JM / 1958 / The heat of denaturation of DNA / J Am Chem Soc 80(11):2911
Ando T / 1966 / A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae / Biochim Biophys Acta 114(1):158~168
Markarian SA / 2006 / Effect of diethylsulfoxide on the thermal denaturation of DNA / Biopolymers 82(1):1~5
Yu ZW / 1994 / Dimethyl sulphoxide: a review of its applications in cell biology / Biosci Rep 14(6):259~281
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UCI(KEPA) : I410-ECN-0101-2016-539-002083113