지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.9
- 수록면
- 171 - 179 (9page)
- DOI
- 10.17519/apiculture.2015.09.30.3.171
이용수
초록· 키워드
오류제보하기
Black Queen Cell Virus (BQCV) is one of the most prevalent viruses in honeybee, and could not be recognized by any infected symptoms in the adult bees. Thus, a newly developed method for rapid RNA isolation and cDNA generation from honeybee samples will be useful and easy to evaluate the presence of BQCV. A significant improvement by only one-step RNA isolation for 10 minutes made possible to detect BQCV RNAs at high quantity. cDNAs were also generated directly from isolated BQCV RNAs by different primer sets for 1 minutes and were subsequently applied to Ultra-Rapid Real-Time PCR by using microchip of 6μl reaction volume with extremely short time in each step of PCR. This system provided the ultra-high speed reaction (30 cycles in less than 10 min) including melting temperature analysis for amplified BQCV products. These results suggest that BQCV detection can be completed within 6 min 42 sec, and the Ultra-Rapid Real-time PCR is sensitive, reliable and time-saving for monitoring BQCV in honeybee.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
LITERATURE CITED
참고문헌 (24)
참고문헌 신청
[학술지(정기간행물)] - Allen, M / 1996 / The incidence and world distribution of honey bee viruses / Bee World 77 : 141 ~ 162
[학술지(정기간행물)] - Anderson, D.L. / 1993 / Pathogens and queen bees / Australian. Beekeeper 94 : 292 ~ 296
[학술지(정기간행물)] - Bailey, L. / 1983 / Association of viruses with two protozoal pathogens of the honey bee / Ann. Appl. Biol 103 : 13 ~ 20
[학술지(정기간행물)] - Bailey, L. / 1977 / Two more small RNA viruses from honey bees and further observations on sacbrood and acute bee-paralysis viruses / J. Gen. Virol 37 : 175 ~ 182
[학술지(정기간행물)] - Bakonyi, T. / 2002 / Phylogenetic analysis of acute bee paralysis virus strains / Appl. Environ. Microbiol 68 : 6446 ~ 6450
[학술지(정기간행물)] - Anderson, D.L. / 1993 / Pathogens and queen bees / Australian. Beekeeper 94 : 292 ~ 296
[학술지(정기간행물)] - Bailey, L. / 1983 / Association of viruses with two protozoal pathogens of the honey bee / Ann. Appl. Biol 103 : 13 ~ 20
[학술지(정기간행물)] - Bailey, L. / 1977 / Two more small RNA viruses from honey bees and further observations on sacbrood and acute bee-paralysis viruses / J. Gen. Virol 37 : 175 ~ 182
[학술지(정기간행물)] - Bakonyi, T. / 2002 / Phylogenetic analysis of acute bee paralysis virus strains / Appl. Environ. Microbiol 68 : 6446 ~ 6450
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