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자료유형
학술저널
저자정보
Yoon-Seok Chang (Seoul National University) Yoon-Keun Kim (Seoul National University) Seong Gyu Jeon (Seoul National University) Sae-Hoon Kim (Seoul National University) Sun-Sin Kim (Seoul National University) Heung-Woo Park (Seoul National University) Kyung-Up Min (Seoul National University) You-Young Kim (National Medical Center) Sang-Heon Cho (Seoul National University)
저널정보
대한면역학회 Immune Network Immune Network Vol.13 No.6
발행연도
2013.12
수록면
295 - 300 (6page)

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초록· 키워드

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Der f 2 is the group 2 major allergen of a house dust mite (Dermatophagoides farinae) and its function has been recently suggested. To determine the optimal condition of sensitization to recombinant Der f 2 (rDer f 2) in murine model of asthma, we compared the effectiveness with different adjuvants in BALB/c and C57BL/6 mice. Mice from both strains sensitized with rDer f 2 by intraperitoneal injection or subcutaneous injection on days 1 and 14. The dosage was 20μg. Freund’s adjuvants with pertussis toxin (FP) or alum alone were used as adjuvants. On days 28, 29, and 30, mice were challenged intranasally with 0.1% rDer f 2. We evaluated airway hyperresponsivenss, eosinophil proportion in lung lavage, airway inflammation, and serum allergen specific antibody responses. Naive mice were used as controls. Airway hyperresponsiveness was increased in C57BL/6 with FP, and BALB/c with alum (PC200: 13.5±6.3, 13.2±6.7 vs. >50 mg/ml, p<0.05). The eosinophil proportion was increased in all groups; C57BL/6 with FP, BALB/c with FP, C57BL/6 with alum, BALB/c with alum (24.8±3.6, 20.3±10.3, 11.0±6.9, 5.7±2.8, vs. 0.0±0.0%, p<0.05). The serum allergen specific IgE levels were increased in C57BL/6 with FP or alum (OD: 0.8±1.4, 1.1±0.8, vs. 0.0±0.0). C57BL/6 mice were better responders to rDer f 2 and as for adjuvants, Freund’s adjuvant with pertussis toxin was better.

목차

INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES

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UCI(KEPA) : I410-ECN-0101-2016-511-001746048