지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.6
- 수록면
- 89 - 95 (7page)
이용수
초록· 키워드
오류제보하기
Immune cells express toll-like receptors (TLRs) and respond to molecular patterns of various pathogens. CpG motif in bacterial DNA activates innate and acquired immune systems through binding to TLR9 of immune cells. Several studies reported that CpG can directly regulate B cell activation, differentiation, and Ig production. However, the role of CpG in B cell growth and Ig production is not fully understood. In this study, we analyzed the effect of CpG on the kinetics of mouse B cell viability, proliferation, and Igs production. Overall, CpG enhanced mouse B cell growth and production of Igs in a dose-dependent manner. Unlike LPS, 100 nM CpG (high dose) did not support TGF-β1-induced IgA and IgG2b production. Moreover, 100 nM CpG treatment abrogated either LPS-induced IgM or LPS/TGF-β1-induced IgA and IgG2b production, although B cell growth was enhanced by CpG under the same culture conditions. We subsequently found that 10 nM CpG (low dose) is sufficient for B cell growth. Again, 10 nM CpG did not support TGF-β1-induced IgA production but, interestingly enough, supported RA-induced IgA production. Further, 10 nM CpG, unlike 100 nM, neither abrogated the LPS/TGF-β1- nor the LPS/RA-induced IgA production. Taken together, these results suggest that dose of CpG is critical in B cell growth and Igs production and the optimal dose of CpG cooperates with LPS in B cell activation and differentiation toward Igs production.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
Akira, S. / 2006 / Pathogen recognition and innate immunity / Cell 124:783~801
Palm, N. W. / 2009 / Pattern recognition receptors and control of adaptive immunity / Immunol. Rev 227:221~233
Ruprecht, C. R. / 2006 / Toll-like receptor stimulation as a third signal required for activation of human naive B cells / Eur. J. Immunol 36:810~816
Pone, E. J. / 2012 / B cell TLRs and induction of immunoglobulin class-switch DNA recombination / Front Biosci 17:2594~2615
Hardenberg, G. / 2007 / Specific TLR ligands regulate APRIL secretion by dendritic cells in a PKR-dependent manner / Eur. J. Immunol 37:2900~2911
Palm, N. W. / 2009 / Pattern recognition receptors and control of adaptive immunity / Immunol. Rev 227:221~233
Ruprecht, C. R. / 2006 / Toll-like receptor stimulation as a third signal required for activation of human naive B cells / Eur. J. Immunol 36:810~816
Pone, E. J. / 2012 / B cell TLRs and induction of immunoglobulin class-switch DNA recombination / Front Biosci 17:2594~2615
Hardenberg, G. / 2007 / Specific TLR ligands regulate APRIL secretion by dendritic cells in a PKR-dependent manner / Eur. J. Immunol 37:2900~2911
이 논문의 저자 정보
최근 본 자료
댓글(0)
0