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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.12
- 수록면
- 376 - 382 (7page)
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초록· 키워드
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Carbon monoxide (CO) is a cytoprotective and homeostatic molecule with important signaling capabilities in physiological and pathophysiological situations. CO protects cells/tissues from damage by free radicals or oxidative stress. NAD(P)H:quinone oxidoreductase (NQO1) is a highly inducible enzyme that is regulated by the Kelch-like ECH-associated protein 1 (Keap1)/nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) pathway, which is central to efficient detoxification of reactive metabolites and reactive oxygen species (ROS). Methods: We generated NQO1 promoter construct. HepG2 cells were treated with CO Releasing Molecules-2 (CORM-2) or CO gas and the gene expressions were measured by RT-PCR, immunoblot, and luciferase assays. Results: CO induced expression of NQO1 in human hepatocarcinoma cell lines by activation of Nrf2. Exposure of HepG2 cells to CO resulted in significant induction of NQO1 in dose- and time-dependent manners. Analysis of the NQO1 promoter indicated that an antioxidant responsible element (ARE)-containing region was critical for the CO-induced Nrf2-dependent increase of NQO1 gene expression in HepG2 cells. Conclusion: Our results suggest that CO-induced Nrf2 increases the expression of NQO1 which is well known to detoxify reactive metabolites and ROS.
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참고문헌 (40)
참고문헌 신청
1. [학술지(정기간행물)] - Tenhunen R / 1968 / The enzymatic conversion of heme to bilirubin by microsomal heme oxygenase / Proc Natl Acad Sci U S A 61 : 748 ~ 755
2. [학술지(정기간행물)] - Otterbein LE / 2003 / Heme oxygenase-1: unleashing the protective properties of heme / Trends Immunol 24 : 449 ~ 455
3. [학술지(정기간행물)] - Otterbein LE / 2003 / Carbon monoxide suppresses arteriosclerotic lesions associated with chronic graft rejection and with balloon injury / Nat Med 9 : 183 ~ 190
4. [학술지(정기간행물)] - Fujimoto H / 2004 / Carbon monoxide protects against cardiac ischemia--reperfusion injury in vivo via MAPK and Akt--eNOS pathways / Arterioscler Thromb Vasc Biol 24 : 1848 ~ 1853
5. [학술지(정기간행물)] - Bauer I / 2009 / Bench-to-bedside review: Carbon monoxide-from mitochondrial poisoning to therapeutic use / Crit Care 13 : 220 ~
2. [학술지(정기간행물)] - Otterbein LE / 2003 / Heme oxygenase-1: unleashing the protective properties of heme / Trends Immunol 24 : 449 ~ 455
3. [학술지(정기간행물)] - Otterbein LE / 2003 / Carbon monoxide suppresses arteriosclerotic lesions associated with chronic graft rejection and with balloon injury / Nat Med 9 : 183 ~ 190
4. [학술지(정기간행물)] - Fujimoto H / 2004 / Carbon monoxide protects against cardiac ischemia--reperfusion injury in vivo via MAPK and Akt--eNOS pathways / Arterioscler Thromb Vasc Biol 24 : 1848 ~ 1853
5. [학술지(정기간행물)] - Bauer I / 2009 / Bench-to-bedside review: Carbon monoxide-from mitochondrial poisoning to therapeutic use / Crit Care 13 : 220 ~
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