지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.10
- 수록면
- 268 - 280 (13page)
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초록· 키워드
오류제보하기
Dengue virus, which belongs to the Flavivirus genus of the Flaviviridae family, causes fatal dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) with infection risk of 2.5 billion people worldwide. However, approved vaccines are still not available. Here, we explored the immune responses induced by alternating prime-boost vaccination using DNA vaccine, adenovirus, and vaccinia virus expressing E protein of dengue virus type 2 (DenV2). Methods: Following immunization with DNA vaccine (pDE), adenovirus (rAd-E), and/or vaccinia virus (VV-E) expressing E protein, E protein-specific IgG and its isotypes were determined by conventional ELISA. Intracellular CD154 and cytokine staining was used for enumerating CD4+ T cells specific for E protein. E protein-specific CD8+ T cell responses were evaluated by in vivo CTL killing activity and intracellular IFN-γ staining. Results: Among three constructs, VV-E induced the most potent IgG responses, Th1-type cytokine production by stimulated CD4+ T cells, and the CD8+ T cell response. Furthermore, when the three constructs were used for alternating prime-boost vaccination, the results revealed a different pattern of CD4+ and CD8+ T cell responses. i) Priming with VV-E induced higher E-specific IgG level but it was decreased rapidly. ii) Strong CD8+ T cell responses specific for E protein were induced when VV-E was used for the priming step, and such CD8+ T cell responses were significantly boosted with pDE. iii) Priming with rAd-E induced stronger CD4+ T cell responses which subsequently boosted with pDE to a greater extent than VV-E and rAd-E. Conclusion: These results indicate that priming with live viral vector vaccines could induce different patterns of E protein-specific CD4+ and CD8+ T cell responses which were significantly enhanced by booster vaccination with the DNA vaccine. Therefore, our observation will provide valuable information for the establishment of optimal prime-boost vaccination against DenV.
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참고문헌 (51)
참고문헌 신청
Swaminathan S / 2009 / Dengue: recent advances in biology and current status of translational research / Curr Mol Med 9:152~173
Guha-Sapir D / 2005 / Dengue fever: new paradigms for a changing epidemiology / Emerg Themes Epidemiol 2:1
Halstead SB / 2007 / Dengue / Lancet 370:1644~1652
Rothman AL / 2004 / Dengue: defining protective versus pathologic immunity / J Clin Invest 113:946~951
Stephenson JR / 2005 / Understanding dengue pathogenesis: implications for vaccine design / Bull World Health Organ 83:308~814
Guha-Sapir D / 2005 / Dengue fever: new paradigms for a changing epidemiology / Emerg Themes Epidemiol 2:1
Halstead SB / 2007 / Dengue / Lancet 370:1644~1652
Rothman AL / 2004 / Dengue: defining protective versus pathologic immunity / J Clin Invest 113:946~951
Stephenson JR / 2005 / Understanding dengue pathogenesis: implications for vaccine design / Bull World Health Organ 83:308~814
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