The Genetic Characteristics of Multidrug-resistant Acinetobacter baumannii Coproducing 16S rRNA Methylase armA and Carbapenemase OXA-23

Jinsook Lim; Hye Hyun Cho; Semi Kim; Jimyung Kim; Kye Chul Kwon; Jong Woo Park; Sun Hoe Koo

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자료유형: 학술저널

저자정보: Jinsook Lim (Chungnam National University) Hye Hyun Cho (Chungnam National University) Semi Kim (Chungnam National University) Jimyung Kim (Chungnam National University) Kye Chul Kwon (Chungnam National University) Jong Woo Park (Chungnam National University) Sun Hoe Koo (Chungnam National University)

저널정보: 대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY Journal of Bacteriology and Virology 제43권 제1호

발행연도: 2013.3

수록면: 27 - 36 (10page)

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Acinetobacter baumannii is a gram-negative organism reported worldwide as a cause of health-care associated infections. Due to its increasing drug resistance, several studies on coproduction of armA and carbapenemase in South Korea and other parts of the world were reported, which can pose significant therapeutic threat. The aim of this study was to investigate genetic characteristics of multidrug-resistant A. baumannii coproducing armA and carbapenemase and its epidemiological relatedness. Forty-five multidrug resistant (MDR) A. baumannii clinical isolates were collected. Antimicrobial susceptibility was determined by agar dilution, Etest and VITEK 2 system. The presence of 16S rRNA methylase and carbapenemase were analyzed by polymerase chain reaction (PCR) and sequencing. Repetitive element palindromic (REP)-PCR was also performed for epidemiologic investigation. All of A. baumannii isolates harbored bla<SUB>OXA-51</SUB>-like gene and 10 isolates showed an upstream ISAba1. 36 isolates (80%) showed amplification of OXA-23, all of which except one had an upstream ISAba1. 16S rRNA methylase armA was found in 44 isolates with high level resistance to aminoglycosides. The rate of coproduction was found in 36 isolates (80%). All isolates showed dominant two patterns in REP-PCR profile. The prevalence of MDR A. baumannii coproducing OXA-23 and armA was high, which the rate of bla<SUB>OXA-23</SUB> coproduction was also high.

#Acinetobacter baumannii #16S rRNA methylase #Aminoglycoside resistance

참고문헌 (32)

참고문헌 신청

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