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학술저널
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대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY 大韓바이러스學會誌 제25권 제2호
발행연도
1995.12
수록면
189 - 198 (10page)

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Three strains of virus designated CPDV-92A, CPDV-92B and CPDV-93A were isolated from the contagious pustular dermatitis(CPD) lesions of the Korean native goat. The isolates caused cytopathic effects in primary goat testicle cell and vero cell. In direct fluorescent assay using anti- CPDV conjugate, positive reactions were specifically detected in the cytoplasm of the infected cells. All isolates were antigenically related to each other and the reference CPDV strains by cross-neutralization test. In the physicochemical and biological properties, the isolates were not affected by BUDR treatment suggesting the virus with DNA genome. All isolates were resistant to lipid solvents such as ether and chloroform, and labile at pH 3.0 and 8.5, and heat treatment. In millipore filteration, the infectivity was significantly decreased at 220nm in pore size. In electron microscopical examination, the ovoid particles measured 140-260nm in size were observed with a crisscross pattern of surface filaments. In pathogenicity, no clinical signs was manifested in mouse and guinea pig, wherease Korean native goats showed the typical skin lesions following intradermal injection of the virus. The hemorrhagic pocks were present on the chorioallantoic membrane of the embryonated eggs. In SDS-PAGE analysis of structural protein, five major and eight minor bands were detected showing no difference in the band pattem among the isolates. In Western immunoblotting analysis, the sera obtained from the immunized goats reacted specifically with 45K and 37K. The inactivated antigen and tissue culture-attenuated CPDV induced the various degree of humoral and cell-mediated immune responses in the goat. The former generated lower immune response than the latter.

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