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학술저널
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대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY 大韓바이러스學會誌 제23권 제1호
발행연도
1993.6
수록면
69 - 77 (9page)

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Seoul virus, one of the etiological agent of hemorrhagic fever with renal syndrome, was inoculated via intrapulmonary route into suckling and weanling SD rats. Suckling rats 24 hour-old were inoculated with 0.02ml of Seoul virus suspension(10' PFU/ml) and 0.1ml was inoculated into weanling rats(3 week-old). Suckling rats became ill and were moribund by 26 days postinfection while weanling rats developed weight loss and ruffled hair. Various tissues were examined during the courses of infection by indirect immunofluorescence test and Avidin-Biotin Complex(ABC) stain. In suckling rats, the virus antigens were detected in bone marrow from the 4th day after inoculation of virus; the lung, kidney and spleen showed fluorescence from the 7th day; and liver, adrenal gland, thymus, cerebrum, cerebellum showed fluorescence from the 14th day. All tissues had viral antigens at 26th day. In contrast, viral antigen was only in bone marrow of weanling rats. Clinical courses, mean time to death and fatal outcome were age-dependent. So suckling rats showed higher susceptibility than weanling rats. In weanling rats, the site of primary replication was thought to be the bone marrow.

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