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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 1992.12
- 수록면
- 111 - 117 (7page)
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To construct a polio chimeric virus expressing part of antigen of hepatitis B virus, we inserted a fragment of S gene of hepatitis B virus into a region of the gene encoding C3 epitope of poliovirus cDNA shuttle vector. The resulting plasmid designated pSBS-69 or RNA transcript derived from pSBS-69 was used to transfect vero cells. The cytopathic effect was observed in the transfected cells and viruses were purified from cell extract by ultracentrifugation. Rabbits were innoculated with polio/HB chimeric viruses. After boosting, antibodies were isolated frorn blood of the rabbits and purified by protein A affinity chromatography. Immunoenzyme assay showed that the antibodies had specificity with hepatitis B virus surface antigen (HBs Ag). The data suggested that polio/Hepatitis B chimeric virus had immunogenicity against HBs Ag.
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