Vortex tube is an energy separation device using high pressure for dividing hot and cold air with simple geometry. The phenomenon of energy separation in vortex tube is not identified yet. For this reason, most of study about the impact on performance according to the shape has been made by experiments. In this study, we compared 10 factors what we think affecting performance of vortex tube in low pressure condition using Plackett-Burman Design. We calculate and analyze mass flow rate(m), temperature difference at cold exit(ΔT<SUB>c</SUB>), and cooling power(W) using CFD. The analysis for the results is made for the main effects that do not interact with other factors. As a result, we found inlet mass flow rate and ΔT<SUB>c</SUB> have large dependence on nozzle area factors. If nozzle area is wider, mass flow rate become greater. In opposite case, ΔT<SUB>c</SUB> become greater. And we found cooling power has large dependence on vortex generator diameter and tube diameter. Therefore, performance of vortex tube can be improved by appropriately selecting design factors.