Scolicidal Effects of Black Cumin Seed (Nigella sativa) Essential Oil on Hydatid Cysts

Hossein Mahmoudvand; Ebrahim Saedi Dezaki; Farnaz Kheirandish; Behrouz Ezatpour; Sareh Jahanbakhsh; Majid Fasihi Harandi

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자료유형: 학술저널

저자정보: Hossein Mahmoudvand (Research Center for Tropical and Infectious Diseases) Ebrahim Saedi Dezaki (Kerman University of Medical Sciences) Farnaz Kheirandish (Lorestan University of Medical Sciences) Behrouz Ezatpour (Lorestan University of Medical Sciences) Sareh Jahanbakhsh (Research Center for Tropical and Infectious Diseases) Majid Fasihi Harandi (Kerman University of Medical Sciences)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.52 No.6

발행연도: 2014.12

수록면: 653 - 659 (7page)

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Surgery remains the preferred treatment for hydatid cyst (cystic echinococcosis, CE). Various scolicidal agents have been used for inactivation of protoscolices during surgery, but most of them are associated with adverse side effects. The present study aimed to evaluate the in vitro scolicidal effect of Nigella sativa (Ranunculaceae) essential oil and also its active principle, thymoquinone, against protoscolices of hydatid cysts. Protoscolices were aseptically aspirated from sheep livers having hydatid cysts. Various concentrations of the essential oil (0.01-10 mg/ml) and thymoquinone (0.125-1.0 mg/ml) were used for 5 to 60 min. Viability of protoscolices was confirmed by 0.1% eosin staining. Furthermore, the components of the N. sativa essential oil were identified by gas chromatography/mass spectroscopy (GC/MS). Our study revealed that the essential oil of N. sativa at the concentration of 10 mg/ml and its main component, thymoquinone, at the concentration of 1 mg/ml had potent scolicidal activities against protoscolices of Echinococcus granulosus after 10 min exposure. Moreover, thymoquinone (42.4%), p-cymene (14.1%), carvacrol (10.3%), and longifolene (6.1%) were found to be the major components of N. sativa essential oil by GC/MS analysis. The results of this study indicated the potential of N. sativa as a natural source for production of a new scolicidal agent for use in hydatid cyst surgery. However, further studies will be needed to confirm these results by checking the essential oil and its active component in in vivo models.

#Echinococcus granulosus #Ranunculaceae #thymoquinone #cystic echinococcosis

참고문헌 (39)

참고문헌 신청

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