Flavonoids Modulate the Proliferation of Neospora caninum in Glial Cell Primary Cultures

Rosan Barbosa de Matos; Suzana Braga-de-Souza; Bruno Pena Seara Pitanga; Victor Diógenes Amaral da Silva; Erica Etelvina Viana de Jesus; Alexandre Morales Pinheiro; Maria de Fátima Dias Costa; Ramon dos Santos El-Bacha; Cátia Suse de Oliveira Ribeiro; Silvia Lima Costa

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저자정보: Rosan Barbosa de Matos (Universidade Federal da Bahia – UFBA) Suzana Braga-de-Souza (Universidade Federal da Bahia – UFBA) Bruno Pena Seara Pitanga (Universidade Federal da Bahia – UFBA) Victor Diógenes Amaral da Silva (Universidade Federal da Bahia – UFBA) Erica Etelvina Viana de Jesus (Universidade Federal da Bahia – UFBA) Alexandre Morales Pinheiro (Universidade Federal do Recôncavo da Bahia) Maria de Fátima Dias Costa (Universidade Federal da Bahia – UFBA) Ramon dos Santos El-Bacha (Universidade Federal da Bahia – UFBA) Cátia Suse de Oliveira Ribeiro (Universidade Federal da Bahia – UFBA) Silvia Lima Costa (Universidade Federal da Bahia – UFBA)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.52 No.6

발행연도: 2014.12

수록면: 613 - 619 (7page)

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Neospora caninum (Apicomplexa; Sarcocystidae) is a protozoan that causes abortion in cattle, horses, sheep, and dogs as well as neurological and dermatological diseases in dogs. In the central nervous system of dogs infected with N. caninum, cysts were detected that exhibited gliosis and meningitis. Flavonoids are polyphenolic compounds that exhibit antibacterial, antiparasitic, antifungal, and antiviral properties. In this study, we investigated the effects of flavonoids in a well-established in vitro model of N. caninum infection in glial cell cultures. Glial cells were treated individually with 10 different flavonoids, and a subset of cultures was also infected with the NC-1 strain of N. caninum. All of the flavonoids tested induced an increase in the metabolism of glial cells and many of them increased nitrite levels in cultures infected with NC-1 compared to controls and uninfected cultures. Among the flavonoids tested, 3",4"-dihydroxyflavone, 3",4",5,7-tetrahydroxyflavone (luteolin), and 3,3",4",5,6-p entahydroxyflavone (quercetin), also inhibited parasitophorous vacuole formation. Taken together, our findings show that flavonoids modulate glial cell responses, increase NO secretion, and interfere with N. caninum infection and proliferation.

#glia #Neospora caninum #flavonoid #nitric oxide (NO)

참고문헌 (27)

참고문헌 신청

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