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논문 기본 정보

자료유형
학술저널
저자정보
Jongmi Baek (Kangwon National University) Jaesung Lee (Kangwon National University) Kyoungkon Kim (Kangwon National University) Taewoo Kim (Kangwon National University) Daejung Kim (Kangwon National University) Cheonan Kim (Enzymebio) Kanazawa Tsutomu (Kitami Institute of Technology) Sarangowa Ochir (Kitami Institute of Technology) Kooyeon Lee (Kangwon National University) Cheol Ho Park (Kangwon National University) Yong-Jik Lee (Kangwon National University) Myeon Choe (Kangwon National University)
저널정보
대한지역사회영양학회 Nutrition Research and Practice Nutrition Research and Practice Vol.7 No.2
발행연도
2013.4
수록면
96 - 102 (7page)

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Obesity, an intractable metabolic disease, currently has no medical treatment without side effects, so studies have been actively carried out to find natural compounds that have anti-obesity activity with minimum side effects. In this study, the anti-obesity effects of water extracts of seven Capsicum annuum L. varieties being Putgochu (Pca), Oyee gochu (Oca), Kwari putgochu (Kca), Green pepper (Gca), Yellow paprika (Yca), Red paprika (Rca) and Cheongyang gochu (Cca), were examined through the evaluation of lipoprotein lipase (LPL) mRNA expression level in 3T3-L1 cells (mouse pre-adipocytes). After capsaicin elimination by chloroform defatting, freeze-dried powder of Cca was treated to 3T3-L1 cells and anti-obesity effects were examined by determining the LPL mRNA level using the RT-PCR method. Of the primary fractions, only proven fractions underwent secondary and tertiary refractionating to determine anti-obesity effects. From seven different Capsicum annuum L., there was a significant decrease of the LPL mRNA expression level of 50.9% in Cca treatment compared to the control group. A significant decrease of the LPL mRNA expression level was shown in primary fractions (Fr) 5 (36.2% decrease) and 6 (30.5% decrease) of the Cca water extracts. Due to the impurities checked by UPLC chromatography, Fr 5 and 6 were refractionated to determine the LPL mRNA expression level. Treatment of Fr 6-6 (35.8% decrease) and Fr 5-6 (35.3% decrease) showed a significant decrease in the LPL mRNA expression level. When analyzed using UPLC, major compounds of Fr 6-6 and Fr 5-6 were very similar. Subsequently, we refractionated Fr 6-6 and Fr 5-6 to isolate the major peak for structure elucidation. Treatment of Fr 5-6-1 (26.6% decrease) and Fr 6-6-1 (29.7% decrease) showed a significant decrease in the LPL mRNA expression level. Consequently, the fractions may have a possibility to ameliorate obesity through the decrease of the LPL mRNA expression level.

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Abstract
Introduction4)
Materials and Methods
Results
Discussion
References

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