지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Objectives: cDNA microarray is an effective method to snapshot gene expression. Functional clustering of gene expressions can identify herbal medicine mechanisms. Much microarray data is available for various herbal medicines. This study compares regulated genes with herbal medicines to evaluate the nature of the drugs.
Methods: Published microarray data were collected. Total RNAs were prepared from dissociated hippocampal dissociate cultures which were given hypoxic shock in the presence of each herbal medicine. Up- or downregulated genes higher than Global M value 0.5 were selected, clustered in functional groups, and compared with various herbal treatments.
Results: 1. Akt2 was upregulated by Acorus gramineus SOLAND, Arisaema amurense var. serratum N<SUB>AKAI</SUB> and Coptis chinensis FRANCH, and they belong to Araceae herb. 2. Nf-κb1, Cd5, Gnγ7 and Sgne1 were upregulated by Arisaema amurense var. serratum NAKAI, Coptis chinensis F<SUB>RANCH</SUB> and Rheum coreanum N<SUB>AKAI</SUB>. 3. Woohwangcheongsim-won, Sohaphyang-won and Scutellaria baicalensis GEORGI downregulated Scp2 and upregulated Tsc2. Woohwangcheongsim-won and Sohaphyang-won upregulated Hba1 and downregulated Myf6. 4. Sohaphyang-won and Scutellaria baicalensis GEORGI downregulated Slc12a1. 5. Woohwangcheongsim-won and Arisaema amurense var. serratum NAKAI upregulated Rarα, Woohwangcheongsim-won and Coptis chinensis F<SUB>RANCH</SUB> downregulated Rab5a and Pdgfrα, and Woohwangcheongsim-won and Rheum coreanum NAKAI upregulated Plcγ1 and downregulated Pla2g1b and Slc10a1.
Conclusions: By clustering microarray, genes are commonly identified to be either up- or downregulated. These results will provide new information to understand the efficacy of herbal medicines and to classify them at the molecular level.
Methods: Published microarray data were collected. Total RNAs were prepared from dissociated hippocampal dissociate cultures which were given hypoxic shock in the presence of each herbal medicine. Up- or downregulated genes higher than Global M value 0.5 were selected, clustered in functional groups, and compared with various herbal treatments.
Results: 1. Akt2 was upregulated by Acorus gramineus SOLAND, Arisaema amurense var. serratum N<SUB>AKAI</SUB> and Coptis chinensis FRANCH, and they belong to Araceae herb. 2. Nf-κb1, Cd5, Gnγ7 and Sgne1 were upregulated by Arisaema amurense var. serratum NAKAI, Coptis chinensis F<SUB>RANCH</SUB> and Rheum coreanum N<SUB>AKAI</SUB>. 3. Woohwangcheongsim-won, Sohaphyang-won and Scutellaria baicalensis GEORGI downregulated Scp2 and upregulated Tsc2. Woohwangcheongsim-won and Sohaphyang-won upregulated Hba1 and downregulated Myf6. 4. Sohaphyang-won and Scutellaria baicalensis GEORGI downregulated Slc12a1. 5. Woohwangcheongsim-won and Arisaema amurense var. serratum NAKAI upregulated Rarα, Woohwangcheongsim-won and Coptis chinensis F<SUB>RANCH</SUB> downregulated Rab5a and Pdgfrα, and Woohwangcheongsim-won and Rheum coreanum NAKAI upregulated Plcγ1 and downregulated Pla2g1b and Slc10a1.
Conclusions: By clustering microarray, genes are commonly identified to be either up- or downregulated. These results will provide new information to understand the efficacy of herbal medicines and to classify them at the molecular level.
목차
Introduction
Research Target and Method
Results
Discussion
Conclusion
References
참고문헌 (44)
참고문헌 신청
1. [학술지(정기간행물)] - Honig LS / 2000 / Apoptosis and neurologic disease / Am J Med 108 (4) : 317 ~ 330
2. [학술지(정기간행물)] - Rego AC / 2003 / Mitochondrial Dysfunction and Reactive Oxygen Species in Excitotoxicity and Apoptosis: Implications for the Pathogenesis of Neurodegenerative Diseases / Neurochem Res 28 (10) : 1563 ~ 1574
3. [학술지(정기간행물)] - International Human Genome Sequencing Consortium / 2001 / Initial sequencing and analysis of the human genome / Nature 209 : 860 ~ 921
4. [학술지(정기간행물)] - Arabidopsis Genome Initiative / 2000 / Analysis of the genome sequence of the flowering plant Arabidopsis thaliana / Nature 408 : 796 ~ 815
5. [학술지(정기간행물)] - Tanaka TS / 2000 / Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray / Proc Natl Acad Sci USA 97 : 9127 ~ 9132
2. [학술지(정기간행물)] - Rego AC / 2003 / Mitochondrial Dysfunction and Reactive Oxygen Species in Excitotoxicity and Apoptosis: Implications for the Pathogenesis of Neurodegenerative Diseases / Neurochem Res 28 (10) : 1563 ~ 1574
3. [학술지(정기간행물)] - International Human Genome Sequencing Consortium / 2001 / Initial sequencing and analysis of the human genome / Nature 209 : 860 ~ 921
4. [학술지(정기간행물)] - Arabidopsis Genome Initiative / 2000 / Analysis of the genome sequence of the flowering plant Arabidopsis thaliana / Nature 408 : 796 ~ 815
5. [학술지(정기간행물)] - Tanaka TS / 2000 / Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray / Proc Natl Acad Sci USA 97 : 9127 ~ 9132
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2016-519-001075188