지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Chicken eggs undergo various physiological changes during egg maturation. To study genes associated with the egg maturation in pre-ovulation (immature) and post-ovulation (mature), we compared gene expression patterns between in the immature egg and mature egg using RNA sequencing data. Mature and immature eggs were obtained from a Heuksaek Jaerae-jong of Korean native chicken. Total RNAs obtained from the eggs were sequenced by Illumina HiSeq 2000 platform, and the generated sequence reads were mapped to Galgal4 reference sequence assembly using Tuxedo Protocol. From the comparison of the RNA sequencing data, 315 genes were differentially expressed between mature and immature eggs, and 46 genes were only detected in immature egg. Further gene ontology (GO) analysis was performed for the differentially expressed genes using DAVID, showing that 29 and 28 GO terms were independently clustered from mature and immature, respectively. From those clustered GO terms, genes related to germ cell development, sex differentiation and defense response to bacterium were mainly expressed in the immature egg, while genes related to regulation of apoptosis, steroid metabolic process and lipid homeostasis were mainly detected in the mature egg. Our results could contribute to understand egg maturation before and after ovulation, and develop genetic markers for improving egg quality and productivity.
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REFERENCES
참고문헌 (25)
참고문헌 신청
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2. [학술지(정기간행물)] - Braw-Tal R / 2002 / The initiation of follicle growth: The oo- cyte or the somatic cells? / Mol Cell Endocrinol 187 : 11 ~ 18
3. [학술지(정기간행물)] - Chen CC / 1996 / Expression of inhibin alpha and inhibin/activin beta A subunits in the granulosa layer of the large preovulatory follicles of the hen / Biol Reprod 55 : 450 ~ 454
4. [학술지(정기간행물)] - Diaz FJ / 2011 / Early avian follicular development is characterized by changes in transcripts involved in steroidogenesis, paracrine signaling and transcription / Mol Reprod Dev 78 : 212 ~ 223
5. [학술지(정기간행물)] - Elis S / 2008 / Search for the genes involved in oocyte maturation and early embryo development in the hen / BMC Genomics 9 : 110 ~
2. [학술지(정기간행물)] - Braw-Tal R / 2002 / The initiation of follicle growth: The oo- cyte or the somatic cells? / Mol Cell Endocrinol 187 : 11 ~ 18
3. [학술지(정기간행물)] - Chen CC / 1996 / Expression of inhibin alpha and inhibin/activin beta A subunits in the granulosa layer of the large preovulatory follicles of the hen / Biol Reprod 55 : 450 ~ 454
4. [학술지(정기간행물)] - Diaz FJ / 2011 / Early avian follicular development is characterized by changes in transcripts involved in steroidogenesis, paracrine signaling and transcription / Mol Reprod Dev 78 : 212 ~ 223
5. [학술지(정기간행물)] - Elis S / 2008 / Search for the genes involved in oocyte maturation and early embryo development in the hen / BMC Genomics 9 : 110 ~
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UCI(KEPA) : I410-ECN-0101-2016-527-001036703