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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.10
- 수록면
- 316 - 322 (7page)
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초록· 키워드
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Xylanase have been used to convert the polymetric xylan into fermentable sugars from the production of ethanol and xylitol from plant biomass. The aim of this study was to isolate and identify xylanolytic bacterium from herbivore feces and was to used the xylanase for enzymatic hydrolysis of biomass. Xylanolytic strains were isolated from 59 different feces of herbivores from Seoul Grand Park located in Gwacheon Gyeonggi-do. The xylanolytic strains were selected by congo red staining and DNS method. Total 67 strains isolated from the herbivores feces were tested for xylanase activity. Among the strains, H10-1, which has the highest xylanase activity, was isolated from feces of Ceratotherium simum. The H10-1 strain was identified as Bacillus pumilus based on its morphological/biochemical characteristics and partial 16S rDNA gene sequences. Culture conditions of B. pumilus H10- 1 such as initial medium pH, incubation temperature and incubation time were optimized for maximum xylanase production. And also xylanase produced by B. pumilus H10-1 was applied for the saccharification of Miscanthus sacchariflorus cv. ‘Geodae 1’, which was pretreated with 1.5M NaOH. The optimized culture conditions of B. pumilus H10-1 were pH 9, 30oC incubation temperature, and 7 day incubation time, respectively. This xylanase activity under the optimized conditions was 20.4±3.3 IU. The crude xylanase produced by B. pumilus H10-1 was used for the saccharification of xylan derived from pretreated ‘Geodae 1’. The saccharification conditions were 50℃, 200 rpm, and 5 days. Saccharification efficiency of pretreated ‘Geodae 1’ by B. pumilus H10-1 was 8.2%.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHOD
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (24)
참고문헌 신청
1. [학술지(정기간행물)] - Sarkar N. / 2012 / Bioethanol production from agricultural wastes: An overview / Renew Energy 37 : 19 ~ 27
2. [학술지(정기간행물)] - Erdei B. / 2013 / Glucose and xylose co-fermentation of pretreated wheat straw using mutants of S. cerevisiae TMB3400 / J. Biotechnol 164 : 50 ~ 58
3. [학술지(정기간행물)] - Tabka M. G. / 2006 / Enzymatic saccharification of wheat straw for bioethanol production by a combined cellulase, xylanase and feruloyl esterase treatment / Enzyme Microbiol. Technol 39 : 897 ~ 902
4. [학술지(정기간행물)] - Ying Xue / 2012 / Split addition of enzymes in enzymatic hydrolysis at high solids concentration to increase sugar concentration for bioethanol production / Journal of Industrial and Engineering Chemistry 18 (2) : 707 ~ 714
5. [학술지(정기간행물)] - Polizeli M. L. T. M. / 2005 / Xylanases from fungi: properties and industrial applications / Appl. Microbiol. Biotechnol 67 : 577 ~ 591
2. [학술지(정기간행물)] - Erdei B. / 2013 / Glucose and xylose co-fermentation of pretreated wheat straw using mutants of S. cerevisiae TMB3400 / J. Biotechnol 164 : 50 ~ 58
3. [학술지(정기간행물)] - Tabka M. G. / 2006 / Enzymatic saccharification of wheat straw for bioethanol production by a combined cellulase, xylanase and feruloyl esterase treatment / Enzyme Microbiol. Technol 39 : 897 ~ 902
4. [학술지(정기간행물)] - Ying Xue / 2012 / Split addition of enzymes in enzymatic hydrolysis at high solids concentration to increase sugar concentration for bioethanol production / Journal of Industrial and Engineering Chemistry 18 (2) : 707 ~ 714
5. [학술지(정기간행물)] - Polizeli M. L. T. M. / 2005 / Xylanases from fungi: properties and industrial applications / Appl. Microbiol. Biotechnol 67 : 577 ~ 591
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