지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.12
- 수록면
- 261 - 266 (6page)
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Environmental pollutants such as polycyclic aromatic hydrocarbons (PAHs) have been implicated in cancer development and progression. However, the effects of PAHs on carcinogenesis are still poorly understood. Here, we characterized a mouse cancer cell line BNL 1ME A. 7R.1 (1MEA) derived by transformation of non-tumorigenic liver cell line BNL CL.2 (BNL) using 3-methylcholanthrene (3MC), a carcinogenic PAH. RT-PCR and immunoblot analysis were used to determine the expression level of mRNA and proteins, respectively. To determine functionality, cell motility was assessed in vitro using a transwell migration assay. Both mRNA and protein levels of E-cadherin were significantly decreased in 1MEA cells in comparison with BNL cells. While the expression levels of mesenchymal markers and related transcription factors were enhanced in 1MEA cells, which could lead to increase in cell motility. Indeed, we found that 7-day exposure of BNL cells to 3-MC reduced the level of the adhesion molecule and epithelial marker Ecadherin and increased reciprocally the level of the mesenchymal marker vimentin in a dose-dependent manner. Taken together, these results indicate that the process of epithelial-mesenchymal transition (EMT) may be activated during premalignant transformation induced by 3-MC. A mechanism study to elucidate the relation between 3-MC exposure and EMT is underway in our laboratory.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (27)
참고문헌 신청
Kim, K. H. / 2013 / A review of airborne polycyclic aromatic hydrocarbons (PAHs) and their human health effects / Environ. Int 60:71~80
Pelclová, D. / 2001 / Biochemical, neuropsychological, and neurological abnormalities following 2,3,7,8-tetrachlorodibenzo-pdioxin (TCDD) exposure / Arch. Environ. Health 56:493~500
Baccarelli, A. / 2002 / Immunologic effects of dioxin: new results from Seveso and comparison with other studies / Environ. Health Perspect 110:1169~1173
Pesatori, A. C. / 2003 / Shortand long-term morbidity and mortality in the population exposed to dioxin after the 'Seveso accident' / Ind. Health 41:127~138
Denison, M. S. / 2002 / Ligand binding and activation of the Ah receptor / Chem. Biol. Interact 141:3~24
Pelclová, D. / 2001 / Biochemical, neuropsychological, and neurological abnormalities following 2,3,7,8-tetrachlorodibenzo-pdioxin (TCDD) exposure / Arch. Environ. Health 56:493~500
Baccarelli, A. / 2002 / Immunologic effects of dioxin: new results from Seveso and comparison with other studies / Environ. Health Perspect 110:1169~1173
Pesatori, A. C. / 2003 / Shortand long-term morbidity and mortality in the population exposed to dioxin after the 'Seveso accident' / Ind. Health 41:127~138
Denison, M. S. / 2002 / Ligand binding and activation of the Ah receptor / Chem. Biol. Interact 141:3~24
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UCI(KEPA) : I410-ECN-0101-2016-513-001047875