Caenorhabditis elegans (C.elegans) holds high potential for a wide range of behavioral and analytic studies. Its genome is composed around twenty thousand of gene, which is similar to human. So, C.elegans is utilized as a model organism for its genetic homology with human. In addition, it has various behavioral properties, for example, chemotaxis, thermotaxis, and electrotaxis. Most previous researches only focused on single-stimulus. But, all organisms live under the condition with combined stimuli. So In this research, we created a device for analyzing the behavior of C.elegans with combined stimuli, temperature and electric field. Furthermore, we compared and analyzed three types or worms, wild type (N2) and two mutant worms (BC347, and CB78), using this device, respectively. We analyzed how the worms react and have preference for a certain stimulus. The device demonstrates feasibility of combined stimulus experiment, which often highly demanded in stimuli studies, we believe that our platform will be set as a standard for combined stimulus device. Therefore, we expect our results to be applied for better studying of human nervous disease.