지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2014.11
- 수록면
- 2,608 - 2,611 (4page)
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초록· 키워드
오류제보하기
Quantification of LDs is one of considerable factor for developing and optimizing bioprocess engineering. Due to the recent development of fluorescence probes, fluorescence based detection techniques were opened a new methodology for realizing time- and labor-free works; high-throughput screening. This report describes a new fluorescence probe for LDs staining; JC-1 (5,5′,6,6′ -Tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide). JC-1 is well-known probe for detecting early stage of apoptosis by measuring mitochondrial membrane potential. We validated that this well-known lipophilic fluorescence probe has a specific interaction with LDs. We optimized staining conditions with JC-1 via systematic variations of concentration, staining time, temperature and solvent concentration. This condition leads to validate on LDs content which was quantitatively monitored using JC-1 and a spectrometer. We also evaluated the possibility for tracking accumulation kinetics of LDs in microalgae through JC-1. Our result presented here confirmed that JC-1 can be a powerful tool for LDs specific fluorescence probe for monitoring kinetics of LDs accumulation.
목차
Abstract
1. 서론
2. 실험방법
3. 실험결과 및 분석
4. 결론
7. 참고문헌
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