Chondrocytes lose their inherent characteristics and shift to fibroblast-like cells on monolayer culture in vitro. Dedifferentiated chondrocytes undergo dramatic phenotypic changes which bring the rearrangement of cytoskeleton and focal adhesion. Cell motility is a harmonized procedure that involves in the changes of cytoskeleton structure and the formation and disassembly of focal adhesion. In this study, we investigate the motile characteristics of the cells with shear stress during dedifferentiation. Dedifferentiated chondrocytes cultured on monolayer have a tendency to move along the direction of shear stress and in proportion to shear strength.