In this study, we propose a new cell-laden process consisting of a dispensing method and a new cross-linking method. A new cell-printed scaffold consisting of cell-embedded alginate struts is designed. The alginate struts are stacked in an interdigitated pattern in successive layers to acquire a three-dimensional (3D) shape. To show the feasibility of the method, we used osteoblasts-like cells. Based on these optimized process conditions, we successfully fabricated a threedimensional, pore structured, cell-laden alginate scaffold. During long cell culture periods, the osteoblasts-like cells in the alginate scaffold survived and proliferated well.