지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.12
- 수록면
- 231 - 239 (9page)
- DOI
- 10.21184/jkeia.2014.12.8.4.231
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초록· 키워드
오류제보하기
Oxidative stress leads to multiple types of cell damage including DNA breaks, mitochondrial failure. Hydrogen peroxide(H₂O₂), a by-product of oxidative stress, has been implicated in triggering apoptosis in various cell types, but there have been no reports on whether H₂O₂ induces apoptosis and p53 expression of human colon cancer cells. P53 is a powerful tumor suppressor gene and is an attractive cancer therapeutic target. A breakthrough in cancer research came from the discovery of the drug which are capable of reactivating p53 function. Most anticancer agents exert their effects by enhancing the anti-proliferative activities of p53. There are evidences that many anti-cancer drugs induce apoptosis through multiple pathways that are at least in part dependent upon p53 activation. The present study investigated the effects of antioxidant vitamin C(VC) and oxidant H₂O₂ on 5-fluorouracil(5-FU) induced p53 expression in human colon cancer HCT-116 cells. Cells were treated with different concentration of 5-FU in combination with H₂O₂, VC. The results demonstrated that 5-FU, H₂O₂, VC did exhibit a significant cytotoxic effect and decreased the cell viability, respectively. The decreased cell viability by H₂O₂ treatment was primarily induced by apoptosis, which was evidenced by TPS assay, FACS analysis. H₂O₂ did exhibit downregulation on 5-FU induced p53 expression. Vitamin C induced p53-dependent apoptosis and enhanced the effects of 5-FU and/or H₂O₂-treated human colon cancer cells to inhibit growth, caused upregulation of p53 expression. These data suggest that optimal VC administration with 5-FU may provide a potentially effective technique in colon cancer therapy.
목차
ABSTRACT
I. Introduction
II. Materials and Methods
III. Results and Discussion
References
참고문헌 (33)
참고문헌 신청
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3. [학술지(정기간행물)] - H. Nagase / 2006 / Structure and function of matrix metalloproteinases and TIMPs / Cardiovascular Res 69 : 562 ~ 573
4. [학술지(정기간행물)] - M. Gomez-Lazaro / 2004 / P53: twenty five years understanding the mechanism of genome protection / Physiol Biochem 60 : 287 ~ 307
5. [학술지(정기간행물)] - T-T. Lim / 2007 / The p53 knowledge base:an integrated information resource for p53research / Oncogene 26 : 1517 ~ 1521
2. [기타자료] - / 2012 / National Cancer Information Center / Year causes of death
3. [학술지(정기간행물)] - H. Nagase / 2006 / Structure and function of matrix metalloproteinases and TIMPs / Cardiovascular Res 69 : 562 ~ 573
4. [학술지(정기간행물)] - M. Gomez-Lazaro / 2004 / P53: twenty five years understanding the mechanism of genome protection / Physiol Biochem 60 : 287 ~ 307
5. [학술지(정기간행물)] - T-T. Lim / 2007 / The p53 knowledge base:an integrated information resource for p53research / Oncogene 26 : 1517 ~ 1521
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