This paper presents a microparticle separator using a spiral microchannel. A particle separator based on the dean vortex was designed, fabricated, and characterized. Two different spiral microchannels were fabricated. Width and initial radius of rotation in the spiral microchannel were fixed to 300 μm and 1.75 mm, respectively. Two different depths of the microchannels were designed at 50 μm and 80 μm. In this experimental study, the equilibrium position of microparticles was monitored by using fluorescent microbeads. In the case of a low dean number (<1.0), lift force and dean drag force were similar, indicating that microbeads were distributed to almost all areas across microchannels. However, in the case of a high dean number (>1.0), dean drag force rather than lift force was dominant, indicating that microbeads moved toward the inner wall of the spiral microchannel.