지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.9
- 수록면
- 180 - 184 (5page)
이용수
초록· 키워드
오류제보하기
Mitochondria play a crucial role in many essential biological events by way of the electron transport chains and intermembrane proteins that they contain. Abnormalities in the mitochondria are strongly correlated with the development of diseases such as atherosclerosis, cancer, and diabetes. However, the study of mitochondria has been referred to as "labor-intensive" because of the difficulty in isolating the organelles from their various sources, which can include cultured cells and tissues. Multiple companies provide mitochondria isolation kits, and it is possible for investigators to use different kits and apply different protocols depending on the source of the mitochondria. Therefore, we focused on producing an isolation buffer that could be applied to both cultured cells and tissues, and optimized an isolation protocol that could be used with both. Specifically, we adjusted the buffer condition that can be applied to human cervical cancer cells, fibroblasts, and tissues such as mouse liver and spleen. We also optimized the protocol to improve the efficacy and efficiency of the steps involved in the isolation of mitochondria. These methodological improvements may contribute to advanced research by allowing investigators to overcome the difficulties involved in isolation of mitochondria from biological samples.
목차
등록된 정보가 없습니다.
참고문헌 (6)
참고문헌 신청
1. [학술지(정기간행물)] - Bratic A / 2014 / The role of mitochondria in aging / J Clin Invest 123 : 951 ~ 957
2. [학술지(정기간행물)] - Frezza C / 2007 / Organelle isolation: functional mitochondria from mouse liver, muscle and cultured fibroblasts / Nat Protoc 2 : 287 ~ 295
3. [학술지(정기간행물)] - Runkel ED / 2014 / Mitochondrial stress: Balancing friend and foe / Exp Gerontol 56 : 194 ~ 201
4. [학술지(정기간행물)] - Schwarz K / 2014 / The breathing heart - Mitochondrial respiratory chain dysfunction in cardiac disease / Int J Cardiol 171 : 134 ~ 143
5. [학술지(정기간행물)] - Sung HJ / 2010 / Mitochondrial respiration protects against from oxygen-associated DNA damage / Nat Commun 1 : 5 ~
2. [학술지(정기간행물)] - Frezza C / 2007 / Organelle isolation: functional mitochondria from mouse liver, muscle and cultured fibroblasts / Nat Protoc 2 : 287 ~ 295
3. [학술지(정기간행물)] - Runkel ED / 2014 / Mitochondrial stress: Balancing friend and foe / Exp Gerontol 56 : 194 ~ 201
4. [학술지(정기간행물)] - Schwarz K / 2014 / The breathing heart - Mitochondrial respiratory chain dysfunction in cardiac disease / Int J Cardiol 171 : 134 ~ 143
5. [학술지(정기간행물)] - Sung HJ / 2010 / Mitochondrial respiration protects against from oxygen-associated DNA damage / Nat Commun 1 : 5 ~
이 논문의 저자 정보
최근 본 자료
댓글(0)
0