지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.9
- 수록면
- 237 - 243 (7page)
이용수
초록· 키워드
오류제보하기
Human Glucagon like peptide-1 (GLP-1) is an incretin hormone that promotes secretion of insulin. In order to eliminate the formation of the soluble aggregate, Ala19 in GLP-1 was substituted with Thr, resulting in a GLP-1 mutant GLP-1A19T. The gene synthesis of GLP-1A19T and the fusion of 6-lysine tagged ubiquitin gene were accomplished by using the overlap extension polymerase chain reaction. The ubiquitin fused GLP-1A19T (K6UbGLP-1A19T) is expressed as form of inclusion body with little formation of the soluble aggregation in recombinant E. coli. In order to produce K6UbGLP-1A19T in large amounts, fed-batch fermentation was carried out in a pH-stat feeding strategy. Maximum dry cell weight of 87.7 g/L and 20.4% of specific K6UbGLP-1A19T content were obtained. Solid-phase refolding using a cation exchanger was carried out to renature K6UbGLP-1A19T. The refolded K6UbGLP-1A19T aggregated little and was released GLP-1A19T by on-column cleavage with ubiquitin-specific protease-1. The molecular mass of GLP-1A19T showed an accurate agreement with its theoretical molecular mass.
목차
Abstract
I. 서론
II. 재료 및 방법
III. 결과 및 고찰
IV. 결론
참고문헌
참고문헌 (25)
참고문헌 신청
Berdichevsky Y / 1999 / Matrix-assisted refolding of single-chain Fv cellulose binding domain fusion proteins / Protein Expression and Purification 17:249~259
Butt TR / 1989 / Ubiquitin fusion augments the yield of cloned gene products in Escherichia coli / Proceedings of the National Academy of Sciences of the United States of America 86:2540~2544
Chang X / 2002 / NMR studies of the aggregation of glucagon-like peptide-1 : formation of a symmetric helical dimer / FEBS Letters 515:165~170
Clark EDB. / 2001 / Protein refolding for industrial processes / Current Opinion in Biotechnology 12:202~207
Einhauer A / 2002 / Expression and purification of homogenous proteins in Saccharomyces cerevisiae based on Ubiquitin-FLAG fusion / Protein Expression and Purification 24:497~504
Butt TR / 1989 / Ubiquitin fusion augments the yield of cloned gene products in Escherichia coli / Proceedings of the National Academy of Sciences of the United States of America 86:2540~2544
Chang X / 2002 / NMR studies of the aggregation of glucagon-like peptide-1 : formation of a symmetric helical dimer / FEBS Letters 515:165~170
Clark EDB. / 2001 / Protein refolding for industrial processes / Current Opinion in Biotechnology 12:202~207
Einhauer A / 2002 / Expression and purification of homogenous proteins in Saccharomyces cerevisiae based on Ubiquitin-FLAG fusion / Protein Expression and Purification 24:497~504
이 논문의 저자 정보
이 논문과 함께 이용한 논문
발암물질인 2-nitrofluorene와 clonidine은 사람지방줄기세포의 sirtuin mRNAs의 발현의 정도를 조절한다
김지연
,
이채영
,
권영호
외 2명
해부·생물인류학
2014
.12
느티만가닥버섯에서 감마선에 의한 돌연변이체들의 유전적 변이
김종봉
,
유동원
생명과학회지
2014
.11
Aspergillus nidulans에서 유성분화 능동조절 유전자 nsdD와 veA 또는 nsdC의 이중결실돌연변이의 분리 및 분석
김이한
,
김하은
,
한동민
미생물학회지
2013
.12
돌연변이 Mannose-binding Lectin 합성과 세포 병리적 연구
장호정
,
정경태
생명과학회지
2013
.03
유해 남조세균 Microcystis aeruginosa의 16S rRNA 및 rpoB 유전자 염기서열 변이 분석
기장서
미생물학회지
2010
.09
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2015-400-002751338