지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.6
- 수록면
- 135 - 139 (5page)
이용수
초록· 키워드
오류제보하기
Pathogenic E. coli associated post-weaning diarrhea (PWD) and edema disease are common diseases in commercially-housed weanling pigs. An enterotoxigenic E. coli (ETEC) oral challenge model has been used to mimic the physiological responses observed in commercial conditions. However, an oral challenge procedure has two major limitations: (1) the ETEC cell density is unknown at the point of oral inoculation, and (2) blending ETEC with traditional TSB (trypticase soy broth) is not palatable and hence decreases acceptability by piglets. Therefore, the purposes of this study were to (1) establish a regression equation that can be used for estimation of ETEC concentration in dilution media using the spectrophotometric measurement of cell density; and (2) examine survival of ETEC after blending either with TSB, sweetener or dextrose. A strain of ETEC (serogroup beta-hemolytic E. coli O149; K91; F4; toxins LT, STa, STb) was grown in TSB for 3.5 hours, centrifuged, the supernatant was discarded, and the ETEC pellet was then blended either with TSB (100 mL), sweetener (60 mL TSB + 40 mL fruit flavored concentrate), or dextrose (50 mL TSB + 50 mL dextrose; 0.5g/mL dextrose). Cell density was measured using the colorimetric method and also plated on a 5% sheep blood agar for counting of ETEC colony forming units at 0, 5, 35, 65 and 125 min after blending. The optical density at 600 nm explained 83% of ETEC colony forming units, indicating that the established linear equation (y= 6E+08x ? 4E+07, P＜0.004) can be used for robust quantification of ETEC cell density in TSB, sweetener and dextrose media. When ETEC was blended with sweetener and dextrose, survival of ETEC was decreased by 45% and 72% within 5 min post-blending. Therefore, further research is required to find out the suitable medium that has potential to improve palatability without compromising survival of ETEC.
목차
Abstract
Ⅰ. Introduction
Ⅱ. Materials and Methods
Ⅲ. Results and Discussion
Ⅳ. Conclusion
References
참고문헌 (8)
참고문헌 신청
Bianch AA / 1999 / Hyperosmotic shock induces the σ32 and σE stress regulons of Escherichia coli / Molecular Microbiology 34:1029~1038
Hampson DJ. / 1994 / Postweaning Escherichia coli diarrhoea in pigs. In : Gyles, C. L. (Ed. ), Escherichia coli in domestic animals and humans, CAB International, pp. 171-192, Guildford, UK / CAB International :171~192
Heo JM / 2010 / Effects of dietary protein level and zinc oxide supplementation on performance responses and gastrointestinal tract characteristics in weaner pigs challenged with an enterotoxigenic strain of Escherichia coli / Animal Production Science 50:827~836
Heo JM / 2013 / Gastrointestinal health and function in weaned pigs : a review of feeding strategies to control post-weaning diarrhoea without using in-feed antimicrobial compounds / Journal of Animal Physiology and Animal Nutrition 97:207~237
Kim JC / 2012 / Nutrition and pathology of weaner pigs : Nutritional strategies to support barrier function in the gastrointestinal tract / Animal Feed Science and Technology 173:3~16
Hampson DJ. / 1994 / Postweaning Escherichia coli diarrhoea in pigs. In : Gyles, C. L. (Ed. ), Escherichia coli in domestic animals and humans, CAB International, pp. 171-192, Guildford, UK / CAB International :171~192
Heo JM / 2010 / Effects of dietary protein level and zinc oxide supplementation on performance responses and gastrointestinal tract characteristics in weaner pigs challenged with an enterotoxigenic strain of Escherichia coli / Animal Production Science 50:827~836
Heo JM / 2013 / Gastrointestinal health and function in weaned pigs : a review of feeding strategies to control post-weaning diarrhoea without using in-feed antimicrobial compounds / Journal of Animal Physiology and Animal Nutrition 97:207~237
Kim JC / 2012 / Nutrition and pathology of weaner pigs : Nutritional strategies to support barrier function in the gastrointestinal tract / Animal Feed Science and Technology 173:3~16
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2015-400-001621726