지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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Staphylococcal enterotoxin B (SEB) is bacterial toxin that induces the activation of immune cells. Because the inhibition of pro-inflammatory effect of SEB can resolve the inflammation, I determined the influence of functional or structural change of SEB on immune cells. The post translational modification of protein occurs through carbamylation. Carbamylation can change the structure of proteins and can modify the biological activity of protein. In the present study, I investigated the effect of carbamylated SEB (CSEB) on the inflammatory response mediated by LPS in HL-60 cells. To determine the anti-inflammatory effect of CSEB, I produced carbamylated SEB using potassium cyanate (KCN) and then examined whether CSEB involved in cytokine releases and apoptosis of LPS-stimulated HL-60 cells. Although CSEB had not any effect on the LPS-stimulated HL-60 cells, the protein levels of IL-8, TNF-α and IL-1β were significantly decreased by CSEB without cytotoxicity. CSEB also blocked Akt and NF-κB activation. These results indicate that the suppressive effect of CSEB in LPS-stimulated cytokine releases is occurred by inhibition of Akt and NF-κB activity. Through further studies, CSEB may be used as anti-inflammatory molecule that makes the immune system more efficient.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (23)
참고문헌 신청
1. [학술지(정기간행물)] - Apostolov EO / 2011 / Endonuclease G mediates endothelial cell death induced by carbamylated LDL / Am J Physiol Heart Circ Physiol 300 : H1997 ~ H2004
2. [학술지(정기간행물)] - Baker BS / 2006 / The role of microorganisms in atopic dermatitis / Clin Exp Immunol 144 : 1 ~ 9
3. [학술지(정기간행물)] - Bianchi R / 2006 / Protective effect of erythropoietin and its carbamylated derivative in experimental Cisplatin peripheral neurotoxicity / Clin Cancer Res 12 : 2607 ~ 2612
4. [학술지(정기간행물)] - Chandrasekaran CV / 2011 / In vitro modulation of LPS/calcimycin induced inflammatory and allergic mediators by pure compounds of Andrographis paniculata (King of bitters) extract / Int Immunopharmacol 11 : 79 ~ 84
5. [학술지(정기간행물)] - 장정현 / 2013 / The Effect of Potassium Cyanate (KCN) on Radiation Treatment of the Colorectal Cancer Cell Line, HCT 116 / 대한의생명과학회지 19 (2) : 98 ~ 104
2. [학술지(정기간행물)] - Baker BS / 2006 / The role of microorganisms in atopic dermatitis / Clin Exp Immunol 144 : 1 ~ 9
3. [학술지(정기간행물)] - Bianchi R / 2006 / Protective effect of erythropoietin and its carbamylated derivative in experimental Cisplatin peripheral neurotoxicity / Clin Cancer Res 12 : 2607 ~ 2612
4. [학술지(정기간행물)] - Chandrasekaran CV / 2011 / In vitro modulation of LPS/calcimycin induced inflammatory and allergic mediators by pure compounds of Andrographis paniculata (King of bitters) extract / Int Immunopharmacol 11 : 79 ~ 84
5. [학술지(정기간행물)] - 장정현 / 2013 / The Effect of Potassium Cyanate (KCN) on Radiation Treatment of the Colorectal Cancer Cell Line, HCT 116 / 대한의생명과학회지 19 (2) : 98 ~ 104
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UCI(KEPA) : I410-ECN-0101-2015-500-001663730