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논문 기본 정보

자료유형
학술저널
저자정보
Chul Hong Kim (Chunnam Techno University) Yong Hwan Kim (Korea Institute of Planning & Evaluation for Technology on Food, Agriculture, Forestry & Fishe) Anne J. Anderson (Utah State University) Young Cheol Kim (Chonnam National University)
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal Vol.30 No.2
발행연도
2014.5
수록면
220 - 227 (8page)

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The GacS/GacA system in the root colonizer Pseudomonas chlororaphis O6 is a key regulator of many traits relevant to the biocontrol function of this bacterium. Proteomic analysis revealed 12 proteins were downregulated in a gacS mutant of P. chlororaphis O6. These GacS-regulated proteins functioned in combating oxidative stress, cell signaling, biosynthesis of secondary metabolism, and secretion. The extent of regulation was shown by real-time RT-PCR to vary between the genes. Mutants of P. chlororaphis O6 were generated in two GacS-regulated genes, trpE, encoding a protein involved in tryptophan synthesis, and prnA, required for conversion of tryptophan to the antimicrobial compound, pyrrolitrin. Failure of the trpE mutant to induce systemic resistance in tobacco against a foliar pathogen causing soft rot, Pectobacterium carotovorum SCCI, correlated with reduced colonization of root surfaces implying an inadequate supply of tryptophan to support growth. Although colonization was not affected by mutation inthe prnA gene, induction of systemic resistance was reduced, suggesting that pyrrolnitrin was an activator of plant resistance as well as an antifungal agent. Study of mutants in the other GacS-regulated proteins will indicate further the features required for biocontrolactivity in this rhizobacterium.

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