The global warming raised the temperature on the sea surface, which resulted in rapid increase in the number of jellyfish. This increase causes damage to marine eco-system, to the fishing equipments and to the product quality and economic loss due to the unwanted inclusion of the jellyfish in targeted fishing. To reduce such damage, we have to know of distribution the biomass of the jellyfish. In recent years, acoustic technology has been extensively used for estimating jellyfish abundance. This acoustic assessment is required for precise estimate of target strength (TS) of a jellyfish. Among methods to estimate TS, theoretical scattering model method is generally dependent on the sound speed contrast (h) and density contrast (g) of jellyfish. So, we measured sound speed contrast and density contrast of Moon jellyfish (Aurelia aurita). The sound speed contrast was measured by using the time-of-flight method. The density contrast was measured by using neutral buoyancy method. The measured sound speed contrast varied between 0.9966 and 1.0031 (mean 0.9999). The measured density contrast varied between 0.9994 and 1.0004 (mean 1.0000). As the moon jellyfish consist mainly of water, they may be similar to seawater in density and sound speed.