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논문 기본 정보

자료유형
학술저널
저자정보
Farshid Mahmodi (University Putra Malaysia) J. B. Kadir (University Putra Malaysia) A. Puteh (University Putra Malaysia) S. S. Pourdad (Dry-land Agricultural Research Institute (DARI)) A. Nasehi (University Putra Malaysia) N. Soleimani (University Putra Malaysia)
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal Vol.30 No.1
발행연도
2014.3
수록면
10 - 24 (15page)

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초록· 키워드

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Genetic diversity and differentiation of 50 Colletotrichum spp. isolates from legume crops studied through multigene loci, RAPD and ISSR analysis. DNA sequence comparisons by six genes (ITS, ACT, Tub2, CHS-1, GAPDH, and HIS3) verified species identity of C. truncatum, C. dematium and C. gloeosporiodes and identity C. capsici as a synonym of C. truncatum. Based on the matrix distance analysis of multigene sequences, the Colletotrichum species showed diverse degrees of intera and interspecific divergence (0.0 to 1.4%) and (15.5-19.9), respectively. A multilocus molecular phylogenetic analysis clustered Colletotrichum spp. isolates into 3 well-defined clades, representing three distinct species; C. truncatum, C. dematium and C. gloeosporioides. The ISSR and RAPD and cluster analysis exhibited a high degree of variability among different isolates and permitted the grouping of isolates of Colletotrichum spp. into three distinct clusters. Distinct populations of Colletotrichum spp. isolates were genetically in accordance with host specificity and inconsistent with geographical origins. The large population of C. truncatum showed greater amounts of genetic diversity than smaller populations of C. dematium and C. gloeosporioides species. Results of ISSR and RAPD markers were congruent, but the effective maker ratio and the number of private alleles were greater in ISSR markers.

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Materials and Methods
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