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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.3
- 수록면
- 7 - 11 (5page)
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초록· 키워드
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Malondialdehyde (MDA), used as an oxidative stress marker, is commonly assayed by measuring the thiobarbituric acid reactive substances (TBARS) using HPLC, as an indicator of the MDA concentration. Since the HPLC method, though highly specific, is time-consuming and expensive, usually it is not suitable for the rapid test in large-scale environmental epidemiologic surveys. The purpose of this study is to develop a simple and rapid method for estimating TBARS levels by using a multiple regression equation that includes TBARS levels measured with a microplate reader as an independent variable. Twelve hour urine samples were obtained from 715 subjects. The concentration of TBARS was measured at three different wavelengths (fluorescence: ?λ<SUB>-ex</SUB> 530 nm and ?λ<SUB>-em</SUB> 550 nm; ?λ<SUB>-ex</SUB> 515 nm and ?λ<SUB>-em</SUB> 553 nm; and absorbance: 532 nm) using microplate reader as well as HPLC. 500 samples were used to develop a regression equation, and the remaining 215 samples were used to evaluate the validity of the regression analysis. The induced multiple regression equation is as follows: TBARS level (?μM) = ?0.282 + 1.830 × (TBARS level measured with a microplate reader at the fluorescence wavelengths ?λ<SUB>-ex</SUB> 530 nm and ?λ<SUB>-em</SUB> 550 nm, ?μM) ?0.685 × (TBARS level measured with a microplate reader at the fluorescence wavelengths ?λ-ex 515 nm and ?λ<SUB>-em</SUB> 553 nm, ?μM) + 0.035 × (TBARS level measured with a microplate reader at the absorbance wavelength 532 nm, ?μM). The estimated TBARS levels showed a better correlation with, and are closer to, the corresponding TBARS levels measured by HPLC compared to the values obtained by the microplate method. The TBARS estimation method reported here is simple and rapid, and that is generally in concordance with HPLC measurements. This method might be a useful tool for monitoring of urinary TBARS level in environmental epidemiologic surveys with large sample sizes.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (8)
참고문헌 신청
Tatum, V. L. / 1990 / Measurement of malondialdehyde by high performance liquid chromatography with fluorescence detection / Lipids 25:226~229
Nair, U. / 2007 / Lipid peroxidationinduced DNA damage in cancer-prone inflammatory diseases:a review of published adduct types and levels in humans / Free Radical Biol. Med 43:1109~1120
Del Rio, D. / 2005 / A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress / Nutr. Metab. Cardiovasc. Dis 15:316~328
Lykkesfeldt, J. / 2001 / Determination of malondialdehyde as dithiobarbituric acid adduct in biological samples by HPLC with fluorescence detection: comparison with ultraviolet-visible spectrophotometry / Clin. Chem 47:1725~1727
Agarwal, R. / 2002 / Rapid, fluorimetric-liquid chromatographic determination of malondialdehyde in biological samples / J. Chromatogr. B, Anal. Technol. Biomed. Life Sci 775:121~126
Nair, U. / 2007 / Lipid peroxidationinduced DNA damage in cancer-prone inflammatory diseases:a review of published adduct types and levels in humans / Free Radical Biol. Med 43:1109~1120
Del Rio, D. / 2005 / A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress / Nutr. Metab. Cardiovasc. Dis 15:316~328
Lykkesfeldt, J. / 2001 / Determination of malondialdehyde as dithiobarbituric acid adduct in biological samples by HPLC with fluorescence detection: comparison with ultraviolet-visible spectrophotometry / Clin. Chem 47:1725~1727
Agarwal, R. / 2002 / Rapid, fluorimetric-liquid chromatographic determination of malondialdehyde in biological samples / J. Chromatogr. B, Anal. Technol. Biomed. Life Sci 775:121~126
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UCI(KEPA) : I410-ECN-0101-2015-510-001343988