지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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This study was designed to identify the effects of Polygoni cuspidati Radix(PCR) on the generation of superoxide anion radicals (ㆍO₂<SUP>-</SUP>), nitric oxide (NO), peroxynitrite (ONOO<SUP>-</SUP>) in the renal epithelial cells of mouse(LLC-PK₁). The effects of PCR on the expression of inflammation-related proteins, IKK-α, phospho-IκB-α, NF-κB (p50, p65), COX-2, iNOS, IL-1β, VCAM-1, were examined by western blotting. For this study, the fluorescent probes, namely dihydrorhodamine 123 (DHR 123), 2’,7’-dichloro dihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) were used. Protein expression levels of IKK-α, phospho-IκB-α, NF-κB (p50, p65), COX-2, iNOS, IL-1β, VCAM-1 were assayed by western blot. PCR reduced H₂O₂-induced cell death dose-dependently. It inhibited the generation of ㆍO2-, NO, ONOO- and PGE2 in the H2O2-treated LLC-PK1 cells in vitro. PCR inhibited the espression of IKK-α, phospho-IκB-α, COX-2, iNOS, IL-1β and VCAM-1 genes by means of decreasing the NF-κB activation. These results suggest that PCR is an effective NO, ㆍO₂<SUP>-</SUP><SUP></SUP>, ONOO<SUP>-</SUP> scavenger, and this substance recommended to be applied in treatment for the inflammatory process and inflammation-related disease.
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참고문헌 (44)
참고문헌 신청
1. [학술지(정기간행물)] - Yu, B.P / 1996 / Aging and oxidative stress: modulation by dietary restriction / Free Radic Biol Med 21 : 651 ~ 668
2. [학술지(정기간행물)] - Helmer, K. S. / 2002 / Induction of NF-ĸB, IĸB-ɑ, and iNOS in rat gastric mucosa during endotoxemia / J Surg Res 104 (1) : 46 ~ 52
3. [학술지(정기간행물)] - Carr, A.C / 2000 / Oxidation of LDL by myeloperoxidase and reactive nitrogen species: reaction pathways and antioxidant protection / Arterioscler Thromb Vasc Biol 20 : 1716 ~ 1723
4. [학술지(정기간행물)] - Haenen, G. R. / 1997 / Peroxynitrite scavenging by flavonoids / Biochem Biophy Res Commu 236 (3) : 591 ~ 593
5. [학술지(정기간행물)] - Kim, H. J. / 2000 / The effct of age on cyclooxygenase-2 gene expression: NF-ĸB activation and IĸBɑ degradation / Free Radic Biol Med 28 (5) : 683 ~ 692
2. [학술지(정기간행물)] - Helmer, K. S. / 2002 / Induction of NF-ĸB, IĸB-ɑ, and iNOS in rat gastric mucosa during endotoxemia / J Surg Res 104 (1) : 46 ~ 52
3. [학술지(정기간행물)] - Carr, A.C / 2000 / Oxidation of LDL by myeloperoxidase and reactive nitrogen species: reaction pathways and antioxidant protection / Arterioscler Thromb Vasc Biol 20 : 1716 ~ 1723
4. [학술지(정기간행물)] - Haenen, G. R. / 1997 / Peroxynitrite scavenging by flavonoids / Biochem Biophy Res Commu 236 (3) : 591 ~ 593
5. [학술지(정기간행물)] - Kim, H. J. / 2000 / The effct of age on cyclooxygenase-2 gene expression: NF-ĸB activation and IĸBɑ degradation / Free Radic Biol Med 28 (5) : 683 ~ 692
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UCI(KEPA) : I410-ECN-0101-2015-510-001222193