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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.2
- 수록면
- 58 - 66 (9page)
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초록· 키워드
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Demand for in vitro pharmacological evaluation and toxicity test using human hepatocytes has been increasing. In USA and Europe, human hepatocytes obtained from donated whole liver unsuitable for transplantation were distributed to researchers and deposited in cell bank facility as cryopreserved vial. In Korea, however, incidence of transplantation-inappropriate whole liver has been quite low and the whole livers almost have so severe liver disease such as fatty or fibrotic liver that cannot meet the demand. In this study we aimed to isolate human hepatocytes from liver resection surgery-originated partial liver, and assure the isolated human hepatocytes and its cryopreserved hepatocytes to be qualified for the in vitro pharmacological evaluation and drug toxicity tests. We compared those with commercially available human hepatocyte, BD GenTest<SUP>TM</SUP> by cell morphology, hepatic gene expression, urea synthesis, albumin secretion, ammonia removal, and cytochrome P450 induction activities. Changes in hepatotoxic gene expression after cryopreservation are evaluated with a typical hepatotoxic drug, acetaminophen. Consequently, the fresh hepatocytes from the partial liver and its cryopreserved hepatocytes expressed their intrinsic hepatic functions well and showed equal hepatotoxicity gene expression trend regardless to cryopreservation. Therefore, liver resection surgery-originated partial liver can be used as a useful source of human hepatocytes for various pharmacological and hepatotoxicity test.
목차
Abstract
1. 서론
2. 재료 및 방법
3. 결과 및 고찰
4. 결론
REFERENCES
참고문헌 (20)
참고문헌 신청
Ramboer, E. / 2013 / Primary hepatocyte cultures as prominent in vitro tools to study hepatic drug transporters / Drug Metab. Rev 45:196~217
Katenz, E. / 2007 / Cryopreservation of primary human hepatocytes: the benefit of trehalose as an additional cryoprotective agent / Liver Transpl 13:38~45
Nishimura, M. / 2007 / Comparison of inducibility of CYP1A and CYP3A mRNAs by prototypical inducers in primary cultures of human, cynomolgus monkey, and rat hepatocytes / Drug Metab Pharmacokinet 22:178~186
Noh, J. K. / 2012 / Quality and availability evaluation of human hepatocytes isolated from rejected partial livers for toxicology and drug metabolism studies in Korea / Proceedings of Conference of Korean Society of Toxicology
Kim, K. A. / 2010 / Assessment of CYP2C19 genetic polymorphisms in a Korean population using a simultaneous multiplex pyrosequencing method to simultaneously detect the CYP2C19*2, CYP2C19*3, and CYP2C19*17alleles / J. Clin. Pharm. Ther 35:697~703
Katenz, E. / 2007 / Cryopreservation of primary human hepatocytes: the benefit of trehalose as an additional cryoprotective agent / Liver Transpl 13:38~45
Nishimura, M. / 2007 / Comparison of inducibility of CYP1A and CYP3A mRNAs by prototypical inducers in primary cultures of human, cynomolgus monkey, and rat hepatocytes / Drug Metab Pharmacokinet 22:178~186
Noh, J. K. / 2012 / Quality and availability evaluation of human hepatocytes isolated from rejected partial livers for toxicology and drug metabolism studies in Korea / Proceedings of Conference of Korean Society of Toxicology
Kim, K. A. / 2010 / Assessment of CYP2C19 genetic polymorphisms in a Korean population using a simultaneous multiplex pyrosequencing method to simultaneously detect the CYP2C19*2, CYP2C19*3, and CYP2C19*17alleles / J. Clin. Pharm. Ther 35:697~703
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UCI(KEPA) : I410-ECN-0101-2015-400-001242930