Expression of Translationally Controlled Tumor Protein (TCTP) Gene of Dirofilaria immitis Guided by Transcriptomic Screening

Yan Fu; Jingchao Lan; Xuhang Wu; Deying Yang; Zhihe Zhang; Huaming Nie; Rong Hou; Runhui Zhang; Wanpeng Zheng; Yue Xie; Ning Yan; Zhi Yang; Chengdong Wang; Li Luo; Li Liu; Xiaobin Gu; Shuxian Wang; Xuerong Peng; Guangyou Yang

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저자정보: Yan Fu (Sichuan Agricultural University) Jingchao Lan (Chengdu Research Base of Giant Panda Breeding) Xuhang Wu (Sichuan Agricultural University) Deying Yang (Sichuan Agricultural University) Zhihe Zhang (Chengdu Research Base of Giant Panda Breeding) Huaming Nie (Sichuan Agricultural University) Rong Hou (Chengdu Research Base of Giant Panda Breeding) Runhui Zhang (Sichuan Agricultural University) Wanpeng Zheng (Sichuan Agricultural University) Yue Xie (Sichuan Agricultural University) Ning Yan (Sichuan Agricultural University) Zhi Yang (Chengdu Research Base of Giant Panda Breeding) Chengdong Wang (Chengdu Research Base of Giant Panda Breeding) Li Luo (Chengdu Research Base of Giant Panda Breeding) Li Liu (Chengdu Research Base of Giant Panda Breeding) Xiaobin Gu (Sichuan Agricultural University) Shuxian Wang (Sichuan Agricultural University) Xuerong Peng (Sichuan Agricultural University) Guangyou Yang (Sichuan Agricultural University)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.52 No.1

발행연도: 2014.2

수록면: 21 - 26 (6page)

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Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.

#Dirofilaria immitis #heartworm #dirofilariasis #translationally controlled tumor protein #transcriptome

참고문헌 (20)

참고문헌 신청

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