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논문 기본 정보

자료유형
학술저널
저자정보
Yong-Kook Kwon (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) Myung Suk Ahn (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) Jong Suk Park (Jeollabuk-do, Agricultural Research and Extension Services) Jang Ryol Liu (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) Dong Su In (Gongju National University) Byung Whan Min (Kyungpook National University) Suk Weon Kim (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
저널정보
고려인삼학회 Journal of Ginseng Research Journal of Ginseng Research Vol.38 No.1
발행연도
2014.1
수록면
52 - 58 (7page)

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초록· 키워드

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To determine whether Fourier transform (FT)-IR spectral analysis combined with multivariate analysis of whole-cell extracts from ginseng leaves can be applied as a high-throughput discrimination system of cultivation ages and cultivars, a total of total 480 leaf samples belonging to 12 categories corresponding to four different cultivars (Yunpung, Kumpung, Chunpung, and an open-pollinated variety) and three different cultivation ages (1 yr, 2 yr, and 3 yr) were subjected to FT-IR. The spectral data were analyzed by principal component analysis and partial least squares-discriminant analysis. A dendrogram based on hierarchical clustering analysis of the FT-IR spectral data on ginseng leaves showed that leaf samples were initially segregated into three groups in a cultivation age-dependent manner. Then, within the same cultivation age group, leaf samples were clustered into four subgroups in a cultivar-dependent manner. The overall prediction accuracy for discrimination of cultivars and cultivation ages was 94.8% in a cross-validation test. These results clearly show that the FT-IR spectra combined with multivariate analysis from ginseng leaves can be applied as an alternative tool for discriminating of ginseng cultivars and cultivation ages. Therefore, we suggest that this result could be used as a rapid and reliable F1 hybrid seed-screening tool for accelerating the conventional breeding of ginseng.

목차

1. Introduction
2. Materials and methods
3. Results
4. Discussion
References

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