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논문 기본 정보

자료유형
학술저널
저자정보
YuKyeong Cho (Pukyong National University) Min-Ji Kim (Pukyong National University) Sung-Koo Kim (Pukyong National University)
저널정보
한국생물공학회 KSBB Journal KSBB Journal 제28권 제6호
발행연도
2013.12
수록면
366 - 371 (6page)

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초록· 키워드

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Ethanol productions were performed by separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes using seaweed, Enteromorpha intestinalis (sea lettuce). Pretreatment conditions were optimized by the performing thermal acid hydrolysis and enzymatic hydrolysis for the increase of ethanol yield. The pretreatment by thermal acid hydrolysis was carried out with different sulfuric acid concentrations in the range of 25 mM to 75 mM H₂SO₄, pretreatment time from 30 to 90 minutes and solid contents of seaweed powder in the range of 10~ 16% (w/v). Optimal pretreatment conditions were determined as 75 mM H₂SO₄ and 13% (w/v) slurry at 121℃ for 60 min. For the further saccharification, enzymatic hydrolysis was performed by the addition of commercial enzymes, Celluclast 1.5 L and Viscozyme L, after the neutralization. A maximum reducing sugar concentration of 40.4 g/L was obtained with 73% of theoretical yield from total carbohydrate. The ethanol concentration of 8.6 g/L of SHF process and 7.6 g/L of SSF process were obtained by the yeast, Saccharomyces cerevisiae KCTC 1126, with the inoculation cell density of 0.2 g dcw/L.

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Abstract
1. Introduction
2. Materials and Methods
3. Results and Discussions
4. Conclusions
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UCI(KEPA) : I410-ECN-0101-2015-400-001299873