In neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease, microglia, a type of brain cells, play critical role as immune cells depending on their activation states. Microglia undergo significant changes in their activation states, which determine their functional roles and migration patterns, upon various physicochemical stimulations from the microenvironment of the brain. Moreover, brain cells can be exposed to exogenously imposed physical stimulations from therapeutic treatments. Therefore, it is important to understand how physical cues influence the activation states of microglia. Our ability to regulate the activation state by physical stimulations can provide new insights for pathological outbreaks of such diseases and may lead us to a novel therapeutic tool. To investigate how physical cues influence the activation states of microglia, we developed a microfluidic platform that can apply the combinatorial stimulation of an fluid shear stress and electric field on microglial cells by controlling two directional cues independently.