Establishment of an Allo-Transplantable Hamster Cholangiocarcinoma Cell Line and Its Application for In Vivo Screening of Anti-Cancer Drugs

Nattapong Puthdee; Kulthida Vaeteewoottacharn; Wunchana Seubwai; Orasa Wonkchalee; Worasak Kaewkong; Amornrat Juasook; Somchai Pinlaor; Chawalit Pairojkul; Chaisiri Wongkham; Seiji Okada; Thidarut Boonmars; Sopit Wongkham

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자료유형: 학술저널

저자정보: Nattapong Puthdee (Khon Kaen University) Kulthida Vaeteewoottacharn (Khon Kaen University) Wunchana Seubwai (Khon Kaen University) Orasa Wonkchalee (Khon Kaen University) Worasak Kaewkong (Naresuan University) Amornrat Juasook (Khon Kaen University) Somchai Pinlaor (Khon Kaen University) Chawalit Pairojkul (Khon Kaen University) Chaisiri Wongkham (Khon Kaen University) Seiji Okada (Kumamoto University) Thidarut Boonmars (Khon Kaen University) Sopit Wongkham (Khon Kaen University)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.51 No.6

발행연도: 2013.12

수록면: 711 - 717 (7page)

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Opisthorchis viverrini (O. viverrini) is a well-known causative agent of cholangiocarcinoma (CCA) in humans. CCA is very resistant to chemotherapy and is frequently fatal. To understand the pathogenesis of CCA in humans, a rodent model was developed. However, the development of CCA in rodents is time-consuming and the xenograft-transplantation model of human CCA in immunodeficient mice is costly. Therefore, the establishment of an in vivo screening model for O. viverrini-associated CCA treatment was of interest. We developed a hamster CCA cell line, Ham-1, derived from the CCA tissue of O. viverrini-infected and N-nitrosodimethylamine-treated Syrian golden hamsters. Ham-1 has been maintained in Dulbecco’s Modified Essential Medium supplemented with 10% fetal bovine serum for more than 30 subcultures. These cells are mostly diploid (2n=44) with some being polyploid. Tumorigenic properties of Ham-1 were demonstrated by allograft transplantation in hamsters. The transplanted tissues were highly proliferative and exhibited a glandular-like structure retaining a bile duct marker, cytokeratin 19. The usefulness of this for in vivo model was demonstrated by berberine treatment, a traditional medicine that is active against various cancers. Growth inhibitory effects of berberine, mainly by an induction of G1 cell cycle arrest, were observed in vitro and in vivo. In summary, we developed the allo-transplantable hamster CCA cell line, which can be used for chemotherapeutic drug testing in vitro and in vivo.

#Opisthorchis viverrini #cholangiocarcinoma #hamster model #allograft transplantation #nti-cancer effect #berberine

참고문헌 (24)

참고문헌 신청

Shin HR / 2010 / Epidemiology of cholangiocarcinoma : an update focusing on risk factors / Cancer Sci 101:579~585

Sripa B / 2008 / Cholangiocarcinoma : lessons from Thailand / Curr Opin Gastroenterol 24:349~356

Khan SA / 2002 / Guidelines for the diagnosis and treatment of cholangiocarcinoma: consensus document / Gut 51(suppl 6):VI1~VI9

Morise Z / 2010 / Surgery and chemotherapy for intrahepatic cholangiocarcinoma / World J Hepatol 2:58~64

Thamavit W / 1978 / Effects of dimethylnitrosamine on induction of cholangiocarcinoma in Opisthorchis viverrini-infected Syrian golden hamsters / Cancer Res 38:4634~4639