Modulation of Antibody Responses against Gnathostoma spinigerum in Mice Immunized with Crude Antigen Formulated in CpG Oligonucleotide and Montanide ISA720

Pewpan M. Intapan; Chakrit Hirunpetcharat; Churairat Kularbkaew; Wiboonchai Yutanawiboonchai; Penchom Janwan; Wanchai Maleewong

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자료유형: 학술저널

저자정보: Pewpan M. Intapan (Khon Kaen University) Chakrit Hirunpetcharat (Mahidol University) Churairat Kularbkaew (Khon Kaen University) Wiboonchai Yutanawiboonchai (Khon Kaen University) Penchom Janwan (Khon Kaen University) Wanchai Maleewong (Khon Kaen University)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.51 No.6

발행연도: 2013.12

수록면: 637 - 644 (8page)

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This study aimed to investigate the antibody responses in mice immunized with Gnathostoma spinigerum crude antigen (GsAg) incorporated with the combined adjuvant, a synthetic oligonucleotide containing unmethylated CpG motif (CpG ODN 1826) and a stable water in oil emulsion (Montanide ISA720). Mice immunized with GsAg and combined adjuvant produced all antibody classes and subclasses to GsAg except IgA. IgG2a/2b/3 but not IgG1 subclasses were enhanced by immunization with CpG ODN 1826 when compared with the control groups immunized with non-CpG ODN and Montanide ISA or only with Montanide ISA, suggesting a biased induction of a Th1-type response by CpG ODN. After challenge infection with live G. spinigerum larvae, the levels of IgG2a/2b/3 antibody subclasses decreased immediately and continuously, while the IgG1 subclass remained at high levels. This also corresponded to a continuous decrease of the IgG2a/IgG1 ratio after infection. Only IgM and IgG1 antibodies, but not IgG2a/2b/3, were significantly produced in adjuvant control groups after infection. These findings suggest that G. spinigerum infection potently induces a Th2-type biased response.

#Gnathostoma spinigerum #CpG oligonucleotide #Th1-type response #Th2-type response #nematode #immunity

참고문헌 (33)

참고문헌 신청

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