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논문 기본 정보

자료유형
학술저널
저자정보
Jung-Mi Kim (Wonkwang University) Suk-Hyun Yun (Chonbuk National University) Seung-Moon Park (Chonbuk National University) Han-Gyu Ko (Forest Mushroom Research Institute) Dae-Hyuk Kim (Chonbuk National University)
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal Vol.29 No.4
발행연도
2013.12
수록면
460 - 464 (5page)

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dsRNA was found in malformed cultures of Lentinula edodes strain FMRI0339, one of the three most popular sawdust cultivated commercial strains of shiitake, and was also found in healthy-looking fruiting bodies and actively growing mycelia. Cloning of the partial genome of the dsRNA revealed the presence of the RdRp sequence of a novel L. edodes mycovirus (LeV), and sequence comparison of the cloned amplicon showed identical sequences sequence to known RNA-dependent RNA polymerase genes of LeV found in strain HKA. The meiotic stability of dsRNA was examined by measuring the ratio of the presence of dsRNA among sexual monokaryotic progeny. More than 40% of the monokaryotic progeny still contained the dsRNA, indicating the persistence of dsRNA during sexual reproduction. Comparing the mycelia growth of monokaryotic progeny suggested that there appeared to be a tendency toward a lower frequency of virus incidence in actively growing progeny.

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