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자료유형
학술저널
저자정보
Jeum Kyu Hong (Gyeongnam National University of Science and Technology) Su Ran Kang (Gyeongnam National University of Science and Technology) Yeon Hwa Kim (Gyeongnam National University of Science and Technology) Dong June Yoon (Gyeongnam National University of Science and Technology) Do Hoon Kim (Gyeongnam National University of Science and Technology) Hyeon Ji Kim (Gyeongnam National University of Science and Technology) Chang Hyun Sung (Gyeongnam National University of Science and Technology) Han Sol Kang (Gyeongnam National University of Science and Technology) Chang Won Choi (Paichai University) Seong Hwan Kim (Dankook University) Young Shik Kim (Sangmyung University)
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal Vol.29 No.4
발행연도
2013.12
수록면
386 - 396 (11page)

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Reactive oxygen species (ROS) generation in tomato plants by Ralstonia solanacearum infection and the role of hydrogen peroxide (H₂O₂) and nitric oxide in tomato bacterial wilt control were demonstrated. During disease development of tomato bacterial wilt, accumulation of superoxide anion (O₂?) and H₂O₂ was observed and lipid peroxidation also occurred in the tomato leaf tissues. High doses of H₂O₂ and sodium nitroprusside (SNP) nitric oxide donor showed phytotoxicity to detached tomato leaves 1 day after petiole feeding showing reduced fresh weight. Both H₂O₂ and SNP have in vitro antibacterial activities against R. solanacearum in a dose-dependent manner, as well as plant protection in detached tomato leaves against bacterial wilt by 10<SUP>6</SUP> and 10<SUP>7</SUP> cfu/ml of R. solanacearum. H₂O₂- and SNP-mediated protection was also evaluated in pots using soil-drench treatment with the bacterial inoculation, and relative ‘area under the disease progressive curve (AUDPC)’ was calculated to compare disease protection by H₂O₂ and/or SNP with untreated control. Neither H₂O₂ nor SNP protect the tomato seedlings from the bacterial wilt, but H₂O₂ + SNP mixture significantly decreased disease severity with reduced relative AUDPC. These results suggest that H₂O₂ and SNP could be used together to control bacterial wilt in tomato plants as bactericidal agents.

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Materials and Methods
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