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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.6
- 수록면
- 129 - 136 (8page)
- DOI
- 10.14815/kjdm.2012.06.39.2.129
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초록· 키워드
오류제보하기
The aim of this study was to develop a new antimicrobial tissue conditioner containing silver nanoparticles (Ag?) and to evaluate its biological performance. Samples of tissue conditioner (Coe-soft, GC cooperation, Tokyo, Japan) containing Ag? with the concentrations of 0 (control), 0.1, 0.5, 1.0, 2.0% were fabricated as 20 x 2.0 (diameter x thickness) ㎜ in size for present experiments. In antimicrobial assay, samples were placed on separate culture plate and microbial suspensions (180 μL) of three tested strains (Staphylococcus aureus, Candida albicans and Escherichia coli) were inoculated. Microbial growth was verified at 24 hours and extended to 72 hours and the antimicrobial effects of samples were evaluated as a percentage of viable cells in withdrawn suspension (100 μL). The borderline of the antimicrobial effect was determined at 99.9 % of microbial reduction. To test the cytotoxicity of the modified tissue conditioner loaded with Ag?, the extracts from experimental samples were prepared according to concentration of Ag? (0, 0.1, 0.5, 1.0, and 2.0%) and incubated at 2, 8, 24 and 72 hours. The cytotoxic effects of the extracts to cultured human gingival fibroblast (HGF) were measured by MTT assay. Samples combined with 0.5, 1.0 and 2.0% Ag? displayed bactericidal effect against three tested strains in 24 and 72 hour incubation time without statistical difference between two incubating periods (p > 0.01). While 0, 0.1 and 0.5% Ag? showed no cytotoxic effect at all of incubating times, significantly high cytotoxic effect (less than 25% cell viability) was revealed at 2.0% Ag? in 24 and 72 hours respectively (p < 0.01) and significantly moderate cytotoxic effect (between 25-50% cell viability) was observed at 1.0% Ag? in 24 and 72 hours respectively and 2.0% at 8 hours (p < 0.01). Within the limitation of this study in vitro, the results suggest that 0.5% Ag? loaded tissue conditioner could be a possible candidate for a novel antimicrobial tissue conditioner with uncompromised cytotoxicity.
목차
ABSTRACT
서론
재료 및 방법
결과
고찰
결론
참고문헌
참고문헌 (33)
참고문헌 신청
1. [학술지(정기간행물)] - 윤제용 / 2005 / 은 이온과의 비교를 통한 나노 은 입자의 항균 특성 연구(050607-050720) / 대한환경공학회지 27 (7) : 771 ~ 776
2. [학술지(정기간행물)] - 이철재 / 2007 / 은나노 입자가 침착된활성탄의 항균특성에 관한 연구 / 대한공업화학회지 18 (4) : 396 ~ 399
3. [학술지(정기간행물)] - Bouillaguet S / 2002 / Long-term cytotoxicity of resin-based dental restorative materials / J Oral Rehabil 29 : 7 ~ 13
4. [학술지(정기간행물)] - Braydich-Stolle L / 2005 / In vitro cytotoxicity of nanoparticles in mammalian germ line stem cells / Toxicol Sci 88 : 412 ~
5. [학술지(정기간행물)] - Bruns W / 1985 / Suppression of intrinsic resistance to penicillins in Staphylococcus aureus, by polidocanol, a dodecyl polyethyleneoxid ether / Antimicrob Agents Chemother 27 : 632 ~ 639
2. [학술지(정기간행물)] - 이철재 / 2007 / 은나노 입자가 침착된활성탄의 항균특성에 관한 연구 / 대한공업화학회지 18 (4) : 396 ~ 399
3. [학술지(정기간행물)] - Bouillaguet S / 2002 / Long-term cytotoxicity of resin-based dental restorative materials / J Oral Rehabil 29 : 7 ~ 13
4. [학술지(정기간행물)] - Braydich-Stolle L / 2005 / In vitro cytotoxicity of nanoparticles in mammalian germ line stem cells / Toxicol Sci 88 : 412 ~
5. [학술지(정기간행물)] - Bruns W / 1985 / Suppression of intrinsic resistance to penicillins in Staphylococcus aureus, by polidocanol, a dodecyl polyethyleneoxid ether / Antimicrob Agents Chemother 27 : 632 ~ 639
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UCI(KEPA) : I410-ECN-0101-2015-510-001117328